| Project/Area Number |
08670699
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Niigata University |
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Principal Investigator |
TANAKA Keiko Niigata University Medical Hospital Assistant, 医学部・附属病院, 助手 (30217020)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJI Shoji Niigata University, Brain Research Institute, Proffessor, 脳研究所, 教授 (70150612)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | paraneoplastic / recombinant HuD protein / cytotoxic T cell / HLA class I / microinjection / paraneoplastic / recombinant HuD protein / cytotoxic T cell / HLA,class I / microiniection / recombinant Yo protein / HLA class I |
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| Research Abstract |
Paraneoplastic sensory neuronopathy (PSN) has been shown to harbor characteristic antineuronal autoantibody "anti-Hu" in their sera and cerebrospinal fluid. Creation of animal models exhibiting clinical or pathological features seen in PSN by means of passive transfer of anti-Hu positive IgG has not been achieved. Although, anti-Hu antibody was shown to induce neuronal cell lysis in vitro, this result has not been reproduced so far. Since prominent T cell infiltration are seen in the central nervous system and posterior spinal ganglion of the patients with anti-Hu syndrome, we studied cytotoxic T cell (CTL) activety in peripheral mononuclear cells from a pantient with PSN harboring anti-Hu antibody. The activated CD8+ T cells from the pantient's venous blood were shown to lyse her own fibroblasts which were incubated with interferon-alpha to induce HLA class I molecules on their surface and the recombinant HuD protein was injected into the cells by microinjector. This is the first report showning the existence of CTL in a patient with PSN.
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