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Interaction between coronary arterial microvessels and cardiac muscles : Analysis during myocardial ischemia and tachcardia.

Research Project

Project/Area Number 08670752
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionTohoku University

Principal Investigator

KOMARU Tatsuya  Tohoku University School of Medicine, The First Department of Ineternal Medicine Research Associate,, 医学部, 助手 (30261530)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsmicrovessels / coronary circulation / myocardial ischemia / G protein / signal transduction / vasodilation / Pertussis toxin / microcirculation / 虚血 / 頻拍 / 筋原性張力 / 冠血流自己調節 / 代謝性因子
Research Abstract

We have established an experimental model to analyze the interaction between cardiac muscle and coronary microcirculation. In that model, excised coronary arterial microvessels and beating hearts were contacted and they could be controlled independently. The coronary arterial microvessel (inner diameter 127-296 mum) was excised from male Japanese white rabbits and was cannulated into a polyethylene tube (outer diameter - 100 mum). When they were immeraed into the water bath with warmed and oxygenated physiological salt solution intrinsic vascular tone was observed without and pharmacological preconstriction. Thereafter, those microvascular preparations were gently embedded onto the surface of beating canine left ventricle perfused by the left anterior descending artery (LAD). The excised microvessels (n=9) on the beating heart were observed by intravital microscope equipped with a floating objective. Changes in the microvascular diameter during the occlusion of LAD was measured with vi … More deomanipulator. The microvessels started to dilate right after the induction of the myocardial ischemia, and the dilation attained significant in 2 minutes (8 (]SY.+-。[)) 2 %). In four minutes, the dilation attained maximum (12 (]SY.+-。[)) 3 %). Subsequently, the diameter began to decrease, and the diameter returned to control level in 30 minutes (1 (]SY.+-。[) 3 %). The transient dilation offers two possibilities in terms of mechanism. First, the dilatory signal from ischemic myocardium may be transient. Second, the vasomotor signals from ischemic myocyte may consist of two components, namely, both vasodilator and constrictor signals which are released sequentially. As some vessels constricted markedly in the later phase of ischemia, the second mechanism is more likely. The experiment to investigate the sensitivity of the vasomotor signal from ischemic heart muscle to pertussis toxin is under way. Although we need to do more experiment, pertussis toxin tended to inhibit the dilation of microvessels during myocardial ischemia. The study using adenosine blocker is planned. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Tatsuya, Komaru et al: "Mechanisis of coronary microvascular dilation induced by the activation of Pertussis toxin-sensitive G protein are vessel size-dependent" Circulation Research. 80. 1-10 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tatsuya, Komaru et al: "Mechanisis of coronary microvascular dilation induced by the activation of Pertussis toxin-sensitive G protein are vessel size-dependent" Circulation. 94. I-242- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akihiko, Sugiyama et al: "The effect of diadenosine tetraphesphate on coronary arteril microvessels." Circulation. 94. I-182- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toshinori, Tanikawa et al: "Role of pretussis toxim-sensitive G protein in active hyperemia of coronary microcirculation" Circulation. 94. I-242- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 小丸 達也 s: "百日咳毒素感受性G蛋白質の刺激による冠微小血管反応のメカニズムnitric oxideとATP感受性カリウムチャネル(K^+ATP)の関与" Japanese Circulation Journal. 61(suppl). 287 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 杉村 彰彦 s: "Diadenosine Tetraphosphote(AP_4A)冠微小動脈径に与える作用とその機序" Japanese Circulation Journal. 61(suppl). 558- (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 小丸 達也 s: "百日咳毒素感受性G蛋白質(Gptx)による冠微小動脈のトーヌス調節" 日本臨床生理学会雑誌. 27(suppl). 60- (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 小丸 達也 s: "虚血心における冠微小血管機能" Therapeutic Reserch. (予定). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tatsuya, Komaru et al: "Rde of ATP-sensitive potassium channel in the regulation of coronary microcirculation" Journal of Molecular Cellular Cardiology. (予定). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tatsuya Komaru et al.: "Mechanisms of coronary microvascular dilation induced by the activation of pertussis toxin-sensitive G protein are vessel size-dependent." Circulation. 94. I-242 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akihiko Sugimura et al.: "The effect of diadenosine tetraphosphate on coronary arterial microvessels." Circulation. 94. I-182 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toshinori Tanikawa et al.: "Role of pertussis toxin-sensitive G protein in active hyperemia of coronary microcirculation." Circulation. 94. I-242 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tatsuya Komaru et al.: "Mechanisms of coronary microvacular responses to the stimulation of pertussis toxin-sensitive G protein : The involvememnt of nitric oxide and ATP-sensitive potassium channels." Japanese Circulation Journal. 61 (Suppl). 287 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tatsuya Komaru et al.: "Mechanisms of coronary microvacular dilation induced by the activation of pertussis toxin-sensitive G protein are vessel size-dependent : Heterogeneous involvement of nitric oxide pathway and ATP-sensitive potassium channels." Circulation Research. 80. 1-10 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akihiko Sugimura et al.: "The effect of diadenosine tetraphosphate (AP4A) on coronary microvascular diameter and its mechanisms." Japanese Circulation Journal. 61 (Suppl). 558 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tatsuya Komaru et al.: "The regulation of coronary microvascular tone by pertussis toxin-sensitive G protein." Japanese Journal of Applied Physiology. 27 (suppl). 60 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tatsuya Komaru et al.: "Coranary microvascular function in the ischemic heart." Therapeutic Research. (planned). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tatsuya Komaru et al: "Role of the ATP-sensitive potassium channel in the regulation of coronary microcirculation : involvement of pertussis toxin-sensitive G protein." Journal of Molecular Cellular Cardiology. (planned). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 小丸 達也: "百日咳毒素感受性G蛋白の刺激による冠微小血管反応のメカニズム-nitric oxideとATP感受性カリウムチャネルの関与" Japanese Circulation Journal. 61(suppl). 287- (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 杉村 彰彦: "Diadenosine Tetraphosphateの冠微小動脈径に与える作用とその機序" Japanese Circulation Journal. 61(suppl). 558- (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 小丸 達也: "百日咳毒素感受性G蛋白による冠微小動脈のトーヌス調節" 日本臨床生理学会雑誌. 27(suppl). 60- (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tatsuya Komaru: "Mechanisms of coronary microvascular dilation inducede by the activatin of pertussis toxin-sensitive G protein are vessel size-dependent" Circulation Research. 80. 1-10 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 小丸 達也: "虚血心における冠微小血管機能" Therapeutic Research. (発表予定). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tatsuya Komaru: "Role of ATP-sensitive potassium chanxel in the regulation of coronary microcirculation" Journal of Molecular Cellular Cardiology. (発表予定). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tatsuya Komaru, Kunio Shirato ら: "Mechanisms of coronary microvascular dilation induced by the activation of pertussis toxin-sensitive G protein are vesselsize-dependent." Circulation Research. 80・1. 1-10 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Akihiko Sugimura, Tatsuya Komaru ら: "The effect of diadenosine tetraphosphate on coronary arterial microvessels." Circulation. 94・8. I-182- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Toshinori Tanikawa, Tatsuya Komaru ら: "Rde of pertussis toxin-sensitive G protein in active hyperemia of coronary micro circulation." Circulation. 94・8. I-242- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tatsuya Komaru, Kunio Shirato ら: "Mechanisms of coronary microvascular dilation induced by the activation of pertussis toxin-sensitive G protein are vessel size-dependent." Circulation. 94・8. I-242- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 小丸達也、谷川俊了 金塚 完、白土邦男ら: "百日咳毒素感受性G蛋白刺激による冠微小血管反応のメカニズム-Nitric oxideとATP感受性カリウムチャネルの関与" Japanese Circulation Journal. 61・Suppl-I (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 杉村彰彦、小丸達也 金塚 完、白土邦男ら: "Diadensine Tetraphosphateの冠微小動脈径に与える作用とその機序" Japanese Circulation Journal. 61・Sullo-I (in press). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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