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Induction of CD4 antigen in B lymphocytes by Epstein-Barr virus infection

Research Project

Project/Area Number 08670844
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

KIKUTA Hideki  Hokkaido Univ.Sch.of Med.Ass.Pro., 医学部, 助教授 (30271654)

Co-Investigator(Kenkyū-buntansha) OKANO Motohiko  Hokkaido Univ.Sch.of Med.Ass., 医学部・附属病院, 助手 (50261300)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
KeywordsEBV / CD4 / HHV-6 / HIV-1 / chemokine / HIV
Research Abstract

1.Fifteen lymphoblastoid cell lines (LCLs) were established by infection of B95-8 Epstein-Barr virus (EBV) in human peripheral blood mononuclear cells. The frequencies of CD4 antigen-positive cells varied among 15 LCL_s(range 0% to 47%).
2.mRNA of CCR5 and CXCR4 was not expressed in 15 LCL_s.
3.One cell line which was expressed CD4 antigen in 90% cells was obtained by the magnetic beads.
4.HHV-6 could not infect 15 LCL_s, indicating that CD4 antigen is not receptor for HHV-6. HIV-1 could replicate in all 15 LCL_s regardless of frequencies of CD4 antigen-positive cells, Indicating that HIV-1 may infect LCL_s by using another receptor.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

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