|Budget Amount *help
¥2,300,000 (Direct Cost : ¥2,300,000)
Fiscal Year 1997 : ¥1,200,000 (Direct Cost : ¥1,200,000)
Fiscal Year 1996 : ¥1,100,000 (Direct Cost : ¥1,100,000)
We have performed the basic research to try to make the novel classification of renal tubular acidosis by analyzing the acid-base related proteins. Initially, we have investigated the expression of acid-base related proteins in the kidney with nephrogenesis. Anti-H-ATPase antibody (Ell), anti-Band III antibody (IVF12), PNA are used for the detection of alphatype intercalated cells and beta type intercalated cells. In the newborn rabbit, nephrogenesis still occurred and cell polality with H-ATPase and Band III were not established. Interestingly, with double labeling of Ell and PNA,both Ell and PNA,only Ell, only PNA labeling cell are (94%, 5%, 1%) in 1 week, (82%, 14%, 4%) in 3weeks, and (82%, 12%, 4%) in adult respectively. It was found that in the newborn premature cells were not well differentiated into alpha and beta type intercalated cells. On carbonic anhydrase (CA) staining, all the differentiating mesenchymal cells contain CA but fade it out with renal maturation. CA supposed to become to localize at proximal cells or intercalated cells with age. On the other hand, clinically several patients with RTA I showed less intense staining of H-ATPase Comparing to normal control. Similarly, some of patients with RTA II showed less intense staining of H-ATpase. In IVF12 staining, the patients studied in this study did not show any lack of immunocytochemical staining which suspect Band III deficiency. Because of the immaturity of cell differentiation and unestblishment of cell polality, newborn infant tended to be acid-base disturbance. Furthermore, with regard to acid-base related proteins, lower production of H-ATPase and Band III may in part relate to RTA.In the future, increment of H-ATPase, Band III,or mRNA of acid-related proteins by acid loading will be required for the better understanding of RTA.