• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Structure and function of human sweat glands.

Research Project

Project/Area Number 08670978
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionSapporo Medical University School of Medicine

Principal Investigator

SAGA Kenji  Sapporo Medical University, Dermatology, Associate Professor, 医学部, 助教授 (10153925)

Co-Investigator(Kenkyū-buntansha) YAMASHITA Toshiharu  Sapporo Medical University, Dermatology, Assistant Professor, 医学部, 講師 (50167706)
三浦 俊祐  札幌医科大学, 医学部, 講師 (50244364)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1998: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordseccrine sweat gland / apocrine sweat gland / extramammary Paget's disease / anionic sites / glycosaminoglycan / epidermal growth factor receptor / atopic dermatitis / sweat secretion / コリン性発汗 / 精神性発汗 / 陰性荷電部位 / シアル酸 / 酵素消化
Research Abstract

Cationic gold labels anionic sites on histological sections. We have previously reported that eccrine and apocrine sweat glands showed completely different distribution of anionic sites and completely different susceptibility to enzyme digestion. Extramammary Paget's disease (EMPD) cells show some characteristics of sweat gland cells. However, it is still not clear whether they differentiate to eccrine or apocrine sweat gland. Therefore, we studied whether EMPD expressed eccrine or apocrine sweat gland-type anionic sites. Cationic gold intensely labeled some EMPD cells at pH 2.0. Neuraminidase completely digested these anionic sites. However, other enzymes did not digest them. These results indicate that sialic acid constitutes anionic sites in Paget's cells. Therefore, EMPD cells possess anionic sites characteristic to normal apocrine sweat gland.
Previous studies have shown that human sweat contains epidermal growth factor (EGF), and that human sweat glands express EGF as well as its … More receptor (EGFR). It is still not clear whether EGFR in human sweat glands is in activated state or in inactivated state. Therefore, we studied the localization of activated EGFR in human eccrine and apocrine sweat glands using a monoclonal antibody that reacts only with the tyrosine phosphorylated (activated) EGFR.In the secretory portion of eccrine sweat glands, nuclei of the secretory cells showed the positive staining with anti-activated EGFR monoclonal antibody. In both coiled and straight portions of eccrine sweat ducts, cytoplasm of luminal cells and nuclei of luminal and peripheral cells were stained with this antibody. In apocrine sweat glands, cytoplasm and nuclei of secretory cells showed the presence of activated EGFR.These data suggest that EGF, produced in the secretory cells of eccrine and apocrine sweat glands, activates EGFR in the secretory cells themselves in autocrine manner. Since ductal cells do not produce EGF, EGF secreted from the secretory cells into sweat activates EGFR in the ductal cells. Therefore, EGF-EGFR system is important to maintain and control the secretion of sweat in the eccrine and apocrine secretory cells. Furthermore, these results support the notion that EGF secreted into sweat regulates the reabsorption of NaCl in the ductal cells of eccrine sweat ducts.
Patients of atopic dermatitis (AD) show many physiological abnormalities of the skin. However, it is still controversial whether they have abnormalities in sweating. We studied emotional and pharmacological sweating in AD and compared with that in healthy subjects. There was no difference in emotional sweating between AD and control subjects. On the other hand, pilocarpine induced sweating was different between AD and control subjects. Sweat rate (volume/time) after pilocarpine stimulation was not significantly different. However, duration of sweating was prolonged in AD.Therefore, total volume of sweat produced by single application of piocarpine was increased in AD. Less

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] 嵯峨賢次: "汗腺と乳房外ページェット病における陰性荷電部位の分布と酵素消化性" 日皮会誌. 106. 1768-1769 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saga K: "Application of cationic colloidal gold staining for the demonstration of anionic sites." Acta Histochem.Cytochem. 31. 1-8 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kato F,Saga K Morimoto Y,Kaneko R: "Pilocarpine-induced cholinergic sweat secretion Compared to emotional sweat secretion in atopic dermatitis" Br J Dermatol. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saga K,Jimbow K: "Histuchemistvy Cytochemistry 1996" The Japan Society of Histochemistry and Cytochemistry, 1105-1106 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 嵯峨賢次: "Day Surgeryの実際" 文光堂, 112-115 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saga K, Jimbow K.: "Extramammary Paget's disease expresses anionic sites characteristic to apocrine sweat gland. In Histochemistry Cytochemistry 1996 (Acta Histochem Cytochem vol 29 supplement), Saito T, editor" The Japan Society of Histochemistry and Cytochemistry. 1105-1106 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saga K: "Application of cationic colloidal gold staining for the demonstration of anionic sites." Acta Histochem Cytochem. 31. 1-8 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kato F, Saga K, Morimoto Y, Kaneko R: "Pilocarpine-induced cholinergic sweat secretion compared to emotional sweat secretion in atopic dermatitis." Br J Dermatol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saga K: "Localization and enzyme digestibility of anionic sites in human sweat glands and extramammary Paget's disease. [in Japanese]" Jpn J Dermatol. 106. 1768-1769 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saga K: Eccrine poroma and its differential diagnosis. In Dermatopathology, Kumakiri M, Miyachi Y, Takigawa M editor. Bunkodo, Tokyo [in Japanese], 136-137 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saga K: Hyperhidrosis, In Practice of Day Surgery, Ohara K, Miyachi Y, Takigawa M editor [in Japanese]. Bunkodo, Tokyo, 93 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saga K: Iontophoresis. In Practice of Day Surgery, Ohara K, Miyachi Y, Takigawa M editor [in Japanese]. Bunkodo, Tokyo, 112-115 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 嵯峨賢次: "汗腺と乳房外ページェット病における陰性荷電部位の分布と酵素消化性" 日皮会誌. 106. 1768-1769 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] Saga K: "Application of cationic colloidal gold staining for the demonstration of amienic sites." Acta Histochem Cytochem. 31. 1-8 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kato F,Saga K,Morimoto Y,Kaneko R: "Pilocarpine-induced cholinergic sweat secretion compared to emotional sweat secretion in atopic clermatitis." Br J Dermatol. 印刷中.

    • Related Report
      1998 Annual Research Report
  • [Publications] Saga K, Jimbow K: "Histochemistry Cytochemistry 1996" The Japan Society of Histochemistry and Cytochemistry, 1105-1106 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] 嵯峨賢次: "Day Surgeryの実際" 文光堂, 112-115 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 嵯峨賢次: "汗腺と乳房外ページェット病における陰性荷電部位の分布と酵素消化性" 日皮会誌. 106. 1768-1769 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Saga K.: "Application of cationic colloidal gold staining for the demonstration of anionic sites." Acta Histochem Cytochem. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Saga K.Jimbow K: "Histochemistry Cytochemistry 1996" The Japan Society of Histochemistry and Cytochemistry, 1105-1106 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 嵯峨賢次: "汗腺と乳房外ページェット病における陰性荷電部位の分布と酵素消化性" 日本皮膚科学会雑誌. 106. 1768-1769 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Saga K,Jimbow K: "Histochemistry Cytochemigtry 1996" The Japan Society of Histochemistry and Cytochemistry, 1105-1106 (1996)

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi