|Budget Amount *help
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1997 : ¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 1996 : ¥1,400,000 (Direct Cost : ¥1,400,000)
To investigate the relationship between the deletion of chromosome 14q32 and grade of malignancy in esophageal cancer, we searched for the deletion of chromosome 14q32, using polymerase chain reation (PCR) and 4microsatellite markers (D14S62, D14S51, D14S267, D14S250).
DNA from 34 patients with esophageal squamous cell carcinoma was examined for loss of heterozygosity (LOH) on chromosome 14q32. Twenty-seven specimens were formalin-fixed and embetted in paraffin and 7 were fresh-frozen and stored at -80ﾟC.
1. The incidence of LOH at at each locus was as follows : 57.1% (8 out of 14 informative cases) at D14S62,35.7% (5 out of 14 informative cases) at D14S267,0% (0 out of 3 informative cases) at D14S250. No informative case was obtained at S14S51.
2. No significant relationship was revealed between LOH and clinicopathological factors (gender, histological type, tumor location, depth of tumor invasion, lymph node metastasis, vascular invasion, histologic stage, surability) and prognosis.
3. Although we could not show prognostic significance of LOH on chromosome 14q32 in esophageal carcinoma in this study, it is hypothesized that the telomeric region of chromosome 14 may contain a tumor suppressor gene, inactivation of which might be caused by chromosomal translocation as well as by allelic loss of this region. Additional investigation of this locus is needed for the evaluation of grade of malignancy in esophageal carcinoma.