|Budget Amount *help
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1997 : ¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 1996 : ¥1,400,000 (Direct Cost : ¥1,400,000)
We study the effects of new ET-K solution in lung preservation, and compare it with EC and UW solutions using an ex vivo rat reperfusion model. The perfusion circuit was initiated by 30 ml of homologous blood. By means of double head roller pump, the blood passed from the venous blood reservoir through the pulmonary artery to be perfused in the examined lung. The lung effluent was returned at the same flow rate to the deoxygenator fresh lung. In fresh group, lung was flushed with saline, and then it was reperfused immediately. In the new ET-K group, UW group and EC group, lung was flushed with the new ET-K,UW,and EC,respectively. The shunt fraction, pulmonary arterial pressure, and peak inspiratory pressure in the new ET-K and UW groups were significantly better than those in the EC group, but were almost equal to those in the fresh group. In addition, this ex vivo rat reperfusion model is simple and highly reliable, and can be widely used in the studies of pulmonary preservation.
amined the effect of db-cAMP against lung injury caused by cold preservation and ischemia-reperfusion. In the fresh group, the lung was flushed with the preservative solution and reperfusion was performed immediately. In the control group and the db-cAMP group, the lung was flushed with either the solution or with a combination of the solution plus db-cAMP,respectively, and preserved at 4ﾟC at 15 hours. The shunt ratios of the reperfused lung in the db-cAMP group were as low as those in the fresh group and significantly lower than those in the control group (p<0.01). Electron microscopic examination showed less damage in te pulmonary arterial endothelium in the db-cAMP group. Immunohistochemical study using anti-rat granulocyte antibody showed less granulocytes in the db-cAMP group. We conclude that db-cAMP attenuates the lung injury by cold preservation and ischemia-reperfuion, at least partly by protection of the vascular endothelium.
上記で開発したモデルを用いて、dibutyryl cyclicAMP(dbcAMP)を保存液に加え、17時間肺保存における効果を調べた。dbcAMPは保存後の肺酸素化能、肺動脈圧、気道内圧などの肺機能を有意に改善することがわかり、電子顕微鏡下の観察でも内皮細胞障害は少なく、さらにanti-rat granulocyte antibodyを用いた免疫染色でもdbcAMPはgranulocyteの凝集を減らし、細胞接着を抑制することが示された。従って、肺再潅流障害の軽減に有効であることが証明された。 Less