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Repair of nerve injury wrapped by encapsulated Schwann cells

Research Project

Project/Area Number 08671579
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionOsaka University

Principal Investigator

SAITOH Youichi  Osaka Univ., Dept.of Neurosurg., Assistant Professor, 医学部, 助手 (20252661)

Co-Investigator(Kenkyū-buntansha) ARITA Norio  Osaka Univ., Dept.of Neurosurg., Associated Professor, 医学部, 講師 (80159508)
HAYAKAWA Toru  Osaka Univ., Dept.of Neurosurg., Professor, 医学部, 教授 (20135700)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsSchwann cell / polymer capsule
Research Abstract

To facilitate the regeneration of crush-injured facial nerve, purified Schwann cells were placed around the crush-injured nerve.
Mature Schwann cells were obtained from dorsal root ganglia of SD rats as described by Kim et al. The two peripheral branches of left facial nerve of each SD rat (10 weeks old) were exposed, and pinched with aneurysm clip (Sugita No.1) for 2 minutes. The evoked electromyograms were recorded by stimulating the proximal and distal portions of the exposed facial nerve (8mA constant current). The mean distance between the two stimulation points was 25mm, and nerve conduction velocity (NCV) was calculated before injury, just after injury, and 14 days after injury. Ten crush-injured portions were covered with collagen gel (0.3ml) containing Schwann cells (1.0*10<@D18@>D1/ml) (Schwann group), and the other 8 were covered with collagen gel only (control group). After electrophysiological studies, the rats were perfused with 4% paraformaldehyde, and the nerves were removed and embedded in paraffin. The 5 mum-thick slices were stained for HE and S-100 protein immunocytochemically. The baseline NCV was 47.6(]SY.+-。[)6.8m/s (mean(]SY.+-。[)SE), and it reduced remarkably just after injury (11.8(]SY.+-。[)2.7m/s). Fourteen days after injury, NCV of Schwann group (30.3(]SY.+-。[)8.6m/s) was significantly greater than that of control group (11.2(]SY.+-。[)3.6m/s). However, immunocytochemistry failed to reveal transplanted Schwann cells around crush-injured nerve.
From these results, transplantation of Schwann cells around crush-injured facial nerve may facilitate the nerve regeneration in the early period after crush-injury. However, it was difficult to determine the substance and mechanism of facilitation of the repair of injured nerves.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Y.Saitoh et al.: "Dose-dependent doxycycline-mediated ACTH secretion from encapsulated Tet-On POMC Neuro2A Cells in the subarachnoid space" Human Gene Therapy. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 齋藤 洋一 他: "Tet-On systemを遺伝子導入した細胞による中枢神経系細胞療法" Biotherapy. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Saitoh et al: "Cell therapy by the cells transfected with gene switch (Tet-On system) in central nervous system" Rest Neurol Neurosci. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Saitoh et al: "Absence of apoptosis in somato tropinomas treated with octreotide" Acta Neurochir. 139. 851-856 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Hagiwara et al: "Transplantation of the xencgeneic cells secreting β-endorphin for pain treatment : Aralysis of the permeability of complements through polymer capsules" Cell Transplantation. 6. 527-530 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Saitoh et al: "Transplantation of the xenogeneic cells sereting β-endorphin for pain treatment : Additional genetic engineoring" Rest Neurol Neurosci. 10. 182-183 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.C.Core et al: "Effects of adrenal medulla transplantation into the third ventricle on the onset of puberty in female rhesus monkeys." Exp Neurol. 140. 172-183 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S M Ekramullarh et al: "The correlation of Ki-67 staiing indices with tumour doubling times in recurrent non-functionig pituitary adenomas." Acta Neurochir. 138. 1449-1455 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Saitoh et al: "Transplantation of the fetal olfactory placode restore reproductive cycles in female rhesus monkeys bearing lesions in the medial basal hypothalamus." Year Book of Endocrinology. 24-25 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Saitoh et al: "Transplantation of the xenogeneic cells secreting beta-endorphin for pain treatment : Additional genetic engineering." Rest Neurol Neurosci. 10. 182-183 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Hiraga et al: "Establishment of spontaneously immortalized rat type 1 astroglial cell lines : The role of p53 in astroglial carcinogenesis." Glia. 18. 185-199 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Taki et al: "Antisense inhibition of the RAD51 enhances radiosensitivity." Biochem Biophys Res Comm. 223. 434-438 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Izumoto et al: "Gene expression of Neural cell adhesion molecule L1 in malignant gliomas and biological significance of L1 glioma invasion." Cancer Research. 56. 1440-1444 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Yamamoto et al: "Abrogation of lung metastasis of human fibrosarcoma cells by ribozyme-mediated suppression of integrinalpha6 subunit expression." Int J Cancer. 65. 519-524 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Hagiwara et al: "Transplantation of the xenogeneic cells secreting beta-endorphin for pain treatment : Analysis of the permeability of complements through polymer capsules." Cell Transplantation. 6. 527-530 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Saitoh et al: "Absence of apoptosis in somatotropinomas treated with octreotide." Acta Neurochir. 139. 851-856 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Ohnishi et al: "Fibronectin-mediated cell migration promotes glioma cell invasion through chemokinetic activity." Clin Exp Metastasis. 15. 538-546 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Saitoh et al: "Cell therapy by the cells transfected with gene switch (Tet-On system) in central nervous system." Rest Neurol Neurosci. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Saitoh et al: "Dose-dependent doxycycline-mediated ACTH secretion from encapsulated Tet-On POMC Neuro2A cells in the subarachnoid space." Human Gene Therapy. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Saitoh et al: "Analgesia induced by transplantation of encapsulated tumor cells secreting beta-endorphin." YEAR BOOK OF PAIN. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Saitoh et al.: "Dose-dependent doxycycline-mediated ACTH secretion from encapsulated Tet-On POMC Neuro2A cells in the subarachnoid space" Human Gene Therapy. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 齋藤洋一他: "Tet-On systemを遺伝子導入した細胞による中枢神経系細胞療法" Bio therapy. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Saitoh et al.: "Cell therapy by the cells transfected with gene switch(Tet-On system)in central nervous system" Rest Neurol Neurosci. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Saitoh et al.: "Absence of apoptosis in somato tropinomas treated with octreotide" Acta Neurochir. 139. 851-856 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Hagiwara et al.: "Transplantation of the xenogeneic cells secreting β-endorphin for pain treatment:Analysis of the pemeability of complements through polymer capsules" Cell Transplantation. 6. 527-530 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Saitoh et al.: "Transplantation of the xensgeneic cells secreting β-endorphin for pain treatment:Additional genetic engineering" Rest Neurol Neurosci. 10. 182-183 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Hagiwara,Y.Saitoh et al: "Transplantation of the xenogeneic cells secreting β-endorphin for pain treatment : Analysis of the permeability of complements through polymer capsules." Cell Transplantation. (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 岡成光、齋藤洋一他: "Pergolide mesilateが著効したしたprolactinomaの一例" ホルモンと臨床 間脳下垂体腫瘍VI. 45. 112-115 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] S M Ekramullarh,Y Saitoh et al: "The correlation of Ki-67 staiing indices with tumour doubling times in recurrent non-functionig pituitary adenomas" Acta Neurochirurgica. 138. 1449-1455 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] A C Gore,Y Saitoh et al: "Effects of adrenal medulla transplantation into the third ventricle on the onset of puberty in female rhesus monkeys." Experimental Neurology. 140. 172-183 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T Uchiyama,Y saitoh et al: "The implant of Schwann cells facilitates the regeneration of crush-injured facial nerve." Restorative Neurology Neuroscience. 9. 54- (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Saitoh et al: "Year Book of Endocrinology" Mosby-Year Book,Inc., 2 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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