|Budget Amount *help
¥2,300,000 (Direct Cost : ¥2,300,000)
Fiscal Year 1998 : ¥800,000 (Direct Cost : ¥800,000)
Fiscal Year 1997 : ¥800,000 (Direct Cost : ¥800,000)
Fiscal Year 1996 : ¥700,000 (Direct Cost : ¥700,000)
We investigated the effect of estrogen as a potential causative for ossification of the posterior longitudinalligament (OPLL). The serum estrogen level was significantly higher in the OPLL patients than controls. Cultured cells derived from OPLL patients had receptors with a higher affinity for estradiol than cells from controls. Cultured cells derived from OPLL patients responded to stimulation by estradiol, elevated the tritiated thymidine uptake and increased the BGP products.
In the study of cell growth factors (TGF beta -1, b-FGF), cultured cells derived from OPLL patients stimulated by estradiol, it was found that productive ratio of TGF beta-1 and b-FGF was increased.
We also studied that proliferative response of peripheral mononuclear cell (PMNC). When PMNC was stimulated by mitogens such as phytohemagglutinin (PHA), pokeweed mitogen (PWM), Staphylococcus aureus Cowan I (SACI), and monoclonal antibody such as antiCD3 mAb, the prolifetative response of PMNC from OPLL patients was lower than that from healthy controls. IL-2 production of T cells stimulated by PHA or antiCD3 mAb was lower in OPLL patients than healthy controls.
And then PMNC stimulated by PHA or anti CD3 mAb with or without TGF beta-1 or b-FGF.Both TGF beta-1 and b-FGF, in addition of higher concentration, it found that the proliferative response was suppressed.
It was studied that proliferative response of CD4 T cells purified from PMNC to anti-CD3 mAb is lower in OPLL patients than in healthy controls, too. And IL-2 production of CD4 T cells was also lower in OPLL patients than in healthy controls.
In the Study of cell surface markers, CD3, CD4, CD8, CD19, CD25 were detected by flow cytometry. It was not found between OPLL group and healthy controls that difference about subpopulation of lymphocyte.