|Budget Amount *help
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1997 : ¥1,100,000 (Direct Cost : ¥1,100,000)
Fiscal Year 1996 : ¥1,000,000 (Direct Cost : ¥1,000,000)
(1)In rabbit, stepwise normovolemic hemodilution model (Hb lO,7,5,3.5gIdl), we measured jejunal mucosal blood flow (Fi) using hydrogen gas clearance technique and jejunal mucosal pH (pHi) using tonometoiy method. to investigate tissue oxygen supply and tissue oxygenation. As a result, pHi remained unchanged until Hb5g/dl, and decreased in Hb3.5g/dl but it was not significant Fi remained unchanged and jejunal mucosal oxygen delivery decreased in parallel with the decrease of arterial oxygen content due to hemodilution. We conclude that jejunal muoosal oxygenation was maintained until Hb5g/dl and this oompensatory mechanism was increase of oxygen uptake.
(2)We performed jejunectomy and end-to-end anastomosis in rabbit model on various degrees of perioperative hemodilution (intraoperative Hb 5g/dl), and investigated the effect of normovolemic hemodilution (NH) on wound healing. Sixteen rabbits were randomized into four groups : Group C=control ; Group HD=NH was performed with hydroxyethyls
tarch (HES) until lib 5g/dl ; Group AA = NH was performed with bled plasma and LIES until Hb 5g/dl ; Group HAT=NH was performed with LIES until Hb 5g/dl and retrasfused after celiotomy. On postoperative day 5(POD5), Tensile strength (TS) on the anastomosis was measured and histological specimen was collected. Time course of measurements of hemoglobin, serum total protein (TP), serum albumin (alb), flbrinogen (Fbg) and blood coagulation factor X[II(F-XIII)were obtained, As a result, Hb was still lower on POD5 in Group HD and AA, but in Group HAT returned lO.lg/dl after retransfusion and returned baseline on 5POD.TS in Group HAT was not significantly different from Group C, but significantly decreased in Group HD and Group AA.TI', aib and Fbg were decreased after NH, returned on 5POD.F-XIII also decreased after NH, but only in Group HAT returened baseline. Histological findings suggested that wound healing was disturbed in Group HD and Group AA.We conclude that intraoperative NH dose not interfere jejunal wound healing, but further investigation must be needed in postoperative maintenance levels of Hb and F-XIII. Less