|Budget Amount *help
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1998 : ¥600,000 (Direct Cost : ¥600,000)
Fiscal Year 1997 : ¥800,000 (Direct Cost : ¥800,000)
Fiscal Year 1996 : ¥700,000 (Direct Cost : ¥700,000)
A simple and reliable polymerase chain reaction-based methods for quantifying human and rat estrogen and PTH/PTHrPR receptor-mRNA are established. Using this method, changen in ERmRNA and PTH/PTHrPR mRNA in rat osteosarcoma cell line (ROS17/2.8) were analyzed. The physiological doses of 1,25(OH)2D^3 significantly increased estrogen receptor(ER)-mRNA, while no significant increase in ERmRNA was detected as a result of stimulation by higher doses of 1,25(OH)2D^3, Western blot analysis of ER protein revealed that ER proteins increased as aresult of stimulation by high dose of 1,25(OH)2D^3, suggesting that 1,25(OH)2D^3 plays an important role of controlling ER in ROS17/2.8. PTH and activin A also increased ERmRNA of ROS17/2.8 cells, but no effect was seen as a result of stimulation by estradiol. On the other hand, estradiol significantly increased PTH/PTHrPR mRNA and cAMP in ROS17/2.8 cells, but 1,25(OH)2D^3. did not show such effect.
Changes in spinal bone mineral density (BMD) before and after menopause were longitudinally investigated in healthy Japanese women. This study clearly demonstrated the postmenopausal women lose their BMD in a biphasic manner. In addition, it was shown that ER gene polymorphism is involved in peak bone mass and bone loss during menopause.