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Immunohistochemical study on cellular proliferative marker and genetic alteration

Research Project

Project/Area Number 08672154
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 病態科学系歯学(含放射線系歯学)
Research InstitutionToyama Medical and Pharmaceutical University

Principal Investigator

IWAI Masayuki  Toyama Medical and Pharmaceutical University, Department of Medicine, Associate Professor, 医学部, 助教授 (20094865)

Co-Investigator(Kenkyū-buntansha) 古田 勲  富山医科薬科大学, 医学部, 教授 (10014268)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
KeywordsInvasive Cancer / Cellular proliferative marker / Proto-oncogene / Immunohistochemistry / p53 / cyclin D1 / p34cdc2 / bcl-2 / び慢性浸潤癌 / p^<53> / Cyclin D1 / p^<34> / び漫性浸潤癌 / サイクリンD_1 / p34 / 細胞増殖 / PCNA / NORs
Research Abstract

A series of studies were performed on the relations of cellular proliferative markers of PCNA, NORs, the genetic expressions of p53, Cyclin D1, p34cdc2, bcl-2 and apoptosis in tongue squamous cell carcinoma (SCC) with the mode of tumor invasion. The specimens from 67 tongue SCCs were used for this project. Immunohistochemical staining was employed to detect PCNA and the expression of genetic proteitns, and siverI staining method employed to examine NORs. The evaluation on those markers was performed by the positive rate of immunostained tumor cells calculated under microscopic field. The mode of tumor invasion was determoned by the method of Yamanoto and Kohama.
Results
1) PCNA was expressed with a lower positive rate (26.5*7.9) in tumors of mode 1-3, and with a high positive rate ( 41.1 * 6.7) in tumors of mode 4
. 2) The numbers of NORs was found with a lower value (3.2*0.8) in tumors of mode 1-3, and with a relatively high value in tumors of mode 4.
3) Cyclin Dl. was, with a relatively … More lower level, expressed in tumors with mode 1-3 (10/24, 41.3%), and with a high level in tumors with mode 4 (12/19, 92.3%).
4) p53 expression was detected with a frequency in 16134 tumors with mode 1-3 (47.0%), and a relatively high frequecy in 9/13 tumors with mode 4 (69.2%).
5) p34cdc2 expression was detected in 24/48 tumors with mode 1-3 (50.0%), and comparatively with a little high frequency in 10/15 tumors with mode 4 (62.7%).
6) The apoptosis was labelled with the value of 2.53 *1.0 in tumors with mode 1-2, with the value of 1.95*1.5 in tumors with mode 3 and with the value of 1.5 * 1.04 in tumors with mode 4.
7) bcl-2 expression was detected in 4/13 tumors with mode 1-2 (30.8%), in 11/27 tumors with mode 3 (40.07%) and in 9/11 tumors with mode 4 (81.8%).
Conclusion
ln tongue SCC, the high level expressions of PCNA, NORs, Cyclin D1, p53, p34cdc2 and bcl-2 were more frequently detected in high invasive tumors. Conversely, apoptosis was labelled with relatively lower value in those tumors. These resuIlts suggest that the genetic abnormalities in relation to cellular proliferative activity involve in tumor invasion. Less

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Yue.LH, Iwai.M, et al.: "Evaluation of argyrophilic nucleolar organizer regions in tongue squamous cell carcinomas." Oral Oncology. 35・1. 70-76 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yao.L, Iwai.M, et al.: "Correlation of bcl-2 and p53 expression with the clinicopathological features in tongue squamous cell carcinomas" Oral Oncology. 35・1. 56-62 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 岳麗華, 岩井正行, 古田勲: "舌癌におけるp34^<cdc2>の免疫組織化学的検討" 口腔腫瘍. 10・3. 99-105 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 岳麗華, 岩井正行 他: "舌癌における核小体形成体(AgNORs)の評価とその臨床的意義" 口腔腫瘍. 10・4. 244-253 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Iwai.M, Yao L, et al.: "Apoptotic cell death in tongue squamous cell carcinoma with special reference to clinicopathological malignancy" Int.J.Oral and maxillofac.Surg.26・1. 266-266 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Itoh S, Iwai M, et al.: "Immunohistochemical study of cyclin D_1 product in tongue squamous cell carcinoma" Int.J.Oral and maxillofac.Surg.26・1. 176-176 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yue, HL,Imai, M,et al.: "Evaluation of argyrophilic nucleolar organizer regions in tohgue squamous cell carcinomas" Oral Oncology. V.35.1. 70-76 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yao L,Imai, M,et al.: "Correlation of bcl-2 and p53 expression with the clinicopathological features in tongue sequamous cell carcinomas" Oral Oncology. V.35.1. 56-62 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yue LH,Imai M,et al.: "Immuno histochemical study on p34^<cdc2> in tengue SCC." Japanese journal of oral tumor. V10.3. 99-105 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yue LH,Imai M,et al.: "Evaluation on AgNoRs and it's clinical significance in tongue SCC." Japanese journal of oral tumor. V10.4. 244-253 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Iwai M,Yao L,et al.: "Apoptotic cell death in tongue squamous cell carcinoma with special reference to clinicopathological malignancy" Int.J.Oral and Maxillofac.Surg.V26.1. 266-266 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Itou S,Iwai, M,et al.: "Immunohistochemical study of cyclin D1 product in tongue squamous cell carcinoma" Int.J.Oral and Maxillofac.Surg.V26.1. 176-176 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yue L H.,Iwai M.et al: "Evaluation of argyrophilic nucleolar Organizer regions in tongue squamous cell Cardinomas" Oral Oncology. 35・1. 70-76 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Lao L,Iwai M.et al: "Correlation of bcl-2 and p53 expression with the clinicopatho-logical features in tongue squanous cell carcinomas" Oral Oncology. 35・1. 56-62 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 岳 麗華、岩井正行、他: "舌癌におけるp3^<4cdc2>の免疫組織化学的検討" 日本口腔腫瘍学会誌. 10・3. 99-105 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 岳 麗華、岩井正行、他: "舌扁平上皮癌におけるArgyrophilic Nucleolar Organizer Regions(AgNORs)の評価とその臨床的意義" 日本口腔腫瘍学会誌. 10・4. 244-253 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Iwai M.Yao L.et al: "Apoptotic cell death in tongue squaorous cell Carcinoma with special reference to clinicopathological malignancy" Oral & Maxillofacial Surgery. 26・1. 266-266 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Itou S,Iwai M,et al: "Immunohistochemical Study of Cyclin D_1 produt in tongue SCC" Oral & Maxillofacial Surgery. 26・1. 176-176 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yao Li,Iwai M, et al: "Clinicopathological Analysis of bcl-2 expression in tongue SCC" Oral & Maxillofacial Surgery. 26・1. 175-176 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Itou S,Iwai M, et al: "Immuaohistochemical Study of Cyclin D_1 Product in tongue SCC" Oral & Maxillofacial Surgery. 26・1. 176-176 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yue L,Iwai M, et al: "Imunohistochemical Study of p34cac2 in tongue squamous cell carcinoma with special reference to Clinicopathological walignancy" Onrl & Maxillofacial Study. 26・1. 263-263 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Iwai M,Yao L, et al: "Apoptotic cell death in tongue squamous cell carcinoma with special reference to clinicopathological malignancy" Oral & Maxillofacial Surgery. 26・1. 266-266 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 岳麗華,岩井正行 他: "舌癌におけるp34の免疫組織化学的検討" 日本口腔腫瘍学会誌. 9・3. 187-187 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 池田昌平,岩井正行 他: "舌扁平上皮癌におけるアポトーシスの免疫組織学的検討" 日本口腔腫瘍学会誌. 9・3. 224-224 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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