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Relationships between diabetes mellitus and oral bacteria in periodontitis.

Research Project

Project/Area Number 08672179
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionTohoku University

Principal Investigator

HORIUCHI Hiroshi  School of Dentistry, Tohoku University Professor, 歯学部, 教授 (00013962)

Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
KeywordsDiabetes mellitus / Periodonto-pathic Bacteria / HL-60 / Experimental periodontitis / Superoxide related enzyme / fibroblast / 糖尿病ラット / ストレプトゾトシン / 一酸化窒素産生酵素 / 実験的糖尿病 / ストレプトゾトシン誘発 / 好中球 / 遊走能 / インシュリン・コントロール / インスリン / 無菌飼育ラット / ストレプトンゾトシン / 普通飼育ラット
Research Abstract

Effects of Blood Glucose Control on Experimental Periodontitis in Streptozotocin-induced Diabetic Rats Effects of Insulin Therapy in Conventional and Germ Free Rats
It was evaluated the effect of the glycemic control on the periodontal condition of streptozotocin-induced diabetic rats with experimental periodontitis. Conventional and germ-free rats were divided into normal rats and insulin-treated rats. An elastic ring was placed around the right mundibular 1St molar. The evaluation of the periodontal condition was made on 14 and 28 days. Blood glucose level, the extent of the inflammatory change, and the quantitative measurement of the alveolar bone area were recorded. The insertion of elastic ring in the periodontal crevice induced attachment loss and alveolar bone resorption. The alveolar bone area of the experimental side in the non-diabetes mellitus group and diabetes mellitus-insulin control group were significantly larger than in the diabetes mellitus group on 14 and 28 days.
Induction of Superoxide related enzymes in HL-60 and human gingival fibroblasts stimulated by periodonto-pathic bacteria
The HL-6O and fibroblasts were incubated with periodonto-pathic bacteria. The HL-60 was evaluated the existence of Superoxide Dismutase and catalase , fibroblasts was estimated by the existence of nitric oxide synthase in each cell. Each enzyme was stained by FITO-laveled indirect-immunological antibody method. The superoxide dismutase and catalase were widely spread in the all cells. The nitric oxide synthase was spread in small area.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] 加藤清: "Streptozotocin誘導糖尿病ラットに対する血糖コントロールが実験的歯周炎に及ぼす影響 1.普通飼育ラットにおけるインスリンの効果" 日本歯周病学会会誌. 38巻・4号. 400-414 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 加藤清: "Streptozotocin誘導糖尿病ラットに対する血糖コントロールが実験的歯周炎に及ぼす影響 2.無菌飼育ラットにおけるインスリンの効果" 日本歯周病学会会誌. 38巻・4号. 415-427 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Kato: "Effects of Blood Glucose Control on Experimental Periodontitis in Streptozotocin-induced Diabetic Rats 1.Effects of Insulin Therapy in Conventional Rats" J.Jpn.Ass.Perio.vol.38, no.4. 400-414 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Kato: "Effects of blood Glucose Control on Experimental Periodontitis in Streptozotocin-induced Diabetic Rats 2.Effects of Insulin Therapy in Germfree Rats" J.Jpn.Ass.Perio.vol.38, no.4. 415-427 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 田中 一美: "歯肉炎症状態の画像解析" 平成8年度 電気関係学会東北支部連合大会抄録集. 58- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 堀内 博: "ハイリスク感染症患者の歯科ケア" 社団法人 仙台歯科医師会, 4 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 堀内 博: "糖尿病: XV 慢性合併症とその対策,歯科疾患" 中山書店(印刷中),

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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