TANAKA Takaaki NIHON UNIVERSITY,School of Dentistry at Matsudo, Crown&Bridge Prosthodontics, Le, 松戸歯学部, 講師 (30207104)
MIZUKAWA Kazuhiro NIHON UNIVERSITY,School of Dentistry at Matsudo, Crown&Bridge Prosthodontics, As, 松戸歯学部, 助教授 (40050025)
|Budget Amount *help
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1997 : ¥1,200,000 (Direct Cost : ¥1,200,000)
Fiscal Year 1996 : ¥900,000 (Direct Cost : ¥900,000)
This study was focused to analyze the induction of delayd-type hypersensitivity (DTH) by the dental adhesive resin monomer. DNFB, which is one of the standard reagents for the induction of DTH, MA and 4-META were used for this investigation. The interaction between bovine serum albumin (BSA) and these reagents was assayd by using nuclear magnetic resonance (NMR) spectroscopy. These reagents were suggested to be haptens, since NMR spectroscopy indicated the chemical shift soon after the mixture of these reagents to BSA solution. However, chemical shifts by MA or 4-META were seen at a relatively lower level compared with that by DNFB.
Ear thickness by the challenge with MA or 4-META showed an increase at an earlier stage (12hr) than a typical pattern with that of DTH such as that of DNFB.Furthremore, The responses to the challenge were different to the haplotype of mice.
The immunohistological study showed a relatively higher frequency of CD4^+T cells in the tissue sections immunized with DNFB, when compared with CD8^+T cells. The detection frequency of CD4^+ and CD8^+T cells were lower in the tissue sections immunized with MA and 4-META than in those with DNFB.Since Enzyme-Linked Immunospot (ELI SPOT) assay indicated that the numbers of polyclonal antibody producing cells in the immunized groups slightly increased in IgM, IgG1, IgG2a, IgG2b, IgG3, and IgA antibodies, it was suggested that theses reagents could have an adjuvant activity. Furthrmore, it was suggested that the humoral immune responses were induced by the immunization with haptens such as DNFB, MA and 4-META, since antigen-specific IgM and IgG_1 immune responses were seen in the immunized group.
各感作群における抗体産生細胞数をEnzyme-Linked Immunospot(ELISPOT) Assayにより検討したところ,ポリクローナルな抗体産生がIgM,IgG1,IgG2a,IgG2b,IgG3,IgAとも若干であるが上昇を示し,Ajuvant活性があることを示唆した.さらに,感作群における特異的抗体産生細胞がIgMおよびIgG1において特に顕著に認められ,Haptenの皮膚感作によって体液性免疫応答が誘導されることが示唆された.