|Budget Amount *help
¥2,200,000 (Direct Cost : ¥2,200,000)
Fiscal Year 1998 : ¥600,000 (Direct Cost : ¥600,000)
Fiscal Year 1997 : ¥900,000 (Direct Cost : ¥900,000)
Fiscal Year 1996 : ¥700,000 (Direct Cost : ¥700,000)
Among proteins of semen origin studies were concentrated on the function, distribution and clinical significance of protein 1, basic fetoprotein, PSA, cystatin C and IgG.
1) Protein 1 (P1)
We have prepared recombinant P1 and demonstrated initially that P1 is a potent inhibitor of phospholipase A2 and phospholipase C.Using monoclonal antibodies, localization of p1 was immunohistochemically demonstrated in Skene's gland as well as prostate. Clinical significance of P1 was further expanded to lung diseases : P1 value in serum was decreased as a result of decreased synthesis at non-ciliated cells in the lung. Furthermore, its synthesis was decreased in patients with cancer with poor prognosis. Generally the value is elevated in BALF in sarcoidosis, in contrast decreased in asthma, indicating that P1 synthesis are associated with Thi and Th2 interregulatory function.
2) Basic fetoprotein
We have initially demonstrated the presence of basic fetoprotein in semen which proved to be structurally identical with that initially purified from placenta. The protein proved to be a new urinary nonspecific inflammatory and tumour marker.
PSA present in urine consists only of free form. Effects of ejaculation and exercise were investigated, Reference intervals were also set. Clinical significance of urinary PSA was none.
4) Cystatin C
Cystatin C rich in semen became a good marker for serum endogenous marker for GER.Clincial significance was under investigation.
Stability of urine IgG was investigated. Urine IgG is of both serum and urine origin, the latter of which was derived from degradation of intact IgG, Fcgamma fragment, digested by proteases in urine.