ヒ素感受性ゲノム解析に基づいたアジア途上国の地下水汚染地域住民のリスク評価
Project/Area Number |
08J07871
|
Research Category |
Grant-in-Aid for JSPS Fellows
|
Allocation Type | Single-year Grants |
Section | 国内 |
Research Field |
Hygiene
|
Research Institution | Shimane University |
Principal Investigator |
阿草 哲郎 島根大学, 医学部, 特別研究員(PD)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2010: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2009: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2008: ¥800,000 (Direct Cost: ¥800,000)
|
Keywords | ヒ素 / ゲノム / 遺伝子多型 / アジア / 環境化学 / 環境毒性学 / リスク評価 / 国際共同研究 |
Research Abstract |
本年度は、ベトナムのRed River Deltaにおけるヒ素汚染地域住民を対象に、purine nucleoside phosphorylase (PNP)の遺伝子多型とヒ素代謝能力の関係について解析した。調査地域の住民334名から、尿と血液を採取した。尿中ヒ素化合物はHPLC/ICP-MSで定量、PNP Gly51Ser多型は、血中から抽出したDNAを用い、PCR-RFLP法で同定した。現在までのところ、Gly/Gly型の尿中DMA/MMAの比がGly/Ser型より有意に高いことが明らかとなった。このことから、Gly/Gly型の方がGly/Ser型よりもモノメチル態からジメチル態へのヒ素の代謝が強いことが示唆された。 また、これまでに調査してきたAS3MTやGSTの遺伝子多型も合わせて、ヒ素曝露依存的な遺伝子多型によるヒ素代謝能の差について検証した。その結果、ヒ素曝露レベルの高い集団において、尿中As[III]/As[V]は、GSTP1 Ile105ValのIle/Val型よりもIle/Ile型で高く、MMA/無機ヒ素は、Ile/Val型よりもIle/Ile型で低かった。つまり、GSTP1 Ile105Valの多型によるヒ素代謝能力の差は、ヒ素曝露依存的に発現することが推察された。 さらに、無機ヒ素を投与した組換え近交系マウス群(計9系統)のヒ素化合物濃度・組成をHPLC-ICP-MSで測定したところ、系統間でヒ素の蓄積と代謝能力に大きな差が認められた。すなわち、C3HやMXH54はヒ素代謝能力が強く、ヒ素を低蓄積する系統で、逆にMXH51やMXH07は代謝能力が弱く、高蓄積する系統であることがわかった。これらの結果を基にした量的形質座位(QTL)解析の結果、第14番染色体にヒ素代謝に関連する感受性遺伝子座を同定することができた。
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Report
(3 results)
Research Products
(58 results)
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[Journal Article] Intake and excretion of arsenic in green turtles (Chelonia mydas) and hawksbill turtles (Eretmochelys imbricata)2011
Author(s)
Agusa, T., Takagi, K., Todd, M.W., Kubota, R., Anan, Y., Iwata, H., Tanabe, S.
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Journal Title
Environmental Chemistry
Volume: 8
Pages: 19-29
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[Journal Article] Stable isotope-guided analysis of biomagnification profiles of arsenic species in a tropical mangrove ecosystem2011
Author(s)
Tu, N.P.C., Agusa, T., Ha, N.N., Tuyen, B.C., Tanabe, S., Takeuchi, I.
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Journal Title
Marine Pollution Bulletin
Volume: 印刷中
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[Journal Article] Accumulation of trace elements in harp seals (Phoca groenlandica) from Pangnirtung in Baffin Island, Canada2011
Author(s)
Agusa, T., Nomura, K., Kunito, T., Anan, Y., Iwata, H., Tanabe, S.
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Journal Title
Marine Pollution Bulletin
Volume: 印刷中
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[Journal Article] 8-Hydroxy-2'-deoxyguanosine and arsenic compounds in urine and serum of a 4-year-old child suffering from acute promyelocytic leukemia during treatment with arsenic trioxide2011
Author(s)
Fujihara, J., Hasegawa, M., Kanai, R., Agusa, T., Iwata, H., Tanabe, S., Yasuda, T., Yamaguchi, S., Takeshita, H.
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Journal Title
Forensic Toxicology
Volume: 印刷中
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[Journal Article] Spatial distribution and corresponding determining factors of metal concentrations in surface sediments of Beppu Bay, southwest Japan2011
Author(s)
Amano, A., Kuwae, M., Agusa, T., Omori, K., Tanabe, S., Takeoka, H., Sugimoto, T.
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Journal Title
Marine Environmental Research
Volume: 印刷中
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[Journal Article] Accumulation features of trace elements in mass-stranded harbor seals (Phocavitulina) in the North Sea coast in 2002 : The body distribution and association with growth and nutrition status2011
Author(s)
Agusa, T., Yasugi, S., Iida, A., Ikemoto, T., Anan, Y., Kuiken, T., Osterhaus, A.D.M.E., Tanabe, S., Iwata, H.
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Journal Title
Marine Pollution Bulletin
Volume: 印刷中
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[Journal Article] Genetic polymorphisms in glutathione S-transferase (GST) superfamily and arsenic metabolism in residents of the Red River Delta, Vietnam2010
Author(s)
Agusa, T., Iwata, H., Fujihara, J., Kunito, T., Takeshita, H., Minh, T.B., Trang, P.T.K., Viet, P.H., Tanabe, S.
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Journal Title
Toxicology and Applied Pharmacology
Volume: 242(3)
Pages: 352-362
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[Journal Article] Global analysis of genetic variation in human arsenic (+3 oxidation state) methyltransferase (AS3MT)2010
Author(s)
Fujihara, J., Soejima, M., Yasuda, T., Koda, Y., Agusa, T., Kunito, T., Tongu, M., Yamada, T., Takeshita, H.
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Journal Title
Toxicology and Applied Pharmacology
Volume: 243(3)
Pages: 292-299
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[Journal Article] Concentrations of trace elements in Meretrix spp.(Mollusca : Bivalva) along the coasts of Vietnam2010
Author(s)
Tu, N.P.C., Ha, N.N., Agusa, T., Ikemoto, T., Tuyen, B.C., Tanabe, S., Takeuchi, I.
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Journal Title
Fisheries Science
Volume: 76(4)
Pages: 677-686
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[Journal Article] Trace elements and stable isotopes (δ^<13>C and δ^<15>N) in whole fish from deep-water of the Sulu Sea and the Celebes Sea2010
Author(s)
Asante, K.W., Agusa, T., Kubota, R., Mochizuki, H., Ramu, K., Subramanian, A., Nishida, S., Ohta, S., Yeh, H-M., Tanabe, S.
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Journal Title
Marine Pollution Bulletin
Volume: 60(9)
Pages: 1560-1570
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[Journal Article] Exposure, metabolism, and health effects of arsenic in residents from arsenic-contaminated groundwater areas of Vietnam and Cambodia : a review2010
Author(s)
Agusa, T., Kunito, T., Kubota, R., Inoue, S., Fujihara, J., Minh, T.B., Ha, N.N., Tu, N.P.C., Trang, P.T.K., Chamnan, C., Takeshita, H., Iwata, H., Tuyen, B.C., Viet, P.H., Tana, T.S., Tanabe, S.
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Journal Title
Reviews on Environmental Health
Volume: 25(3)
Pages: 193-220
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[Journal Article] Genetic polymorphisms in glutathione S-transferase(GST)superfamily and arsenic metabolism in residents of the Red River Delta2010
Author(s)
Agusa, T., Iwata, H., Fujihara, J., Kunito, T., Takeshita, H., Minh, T.B., Trang, P.T.K., Viet, P.H., Tanabe, S.
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Journal Title
Toxicology and Applied Pharmacology 242
Pages: 352-362
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[Journal Article] Global analysis of genetic variation in human arsenic(+3 oxidation state)methyltransferase(AS3MT)2010
Author(s)
Fujihara, J., Soejima, M., Yasuda, T., Koda, Y., Agusa, T., Kunito, T., Tongu, M., Yamada, T., Takeshita, H.
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Journal Title
Toxicology and Applied Pharmacology 243(3)
Pages: 292-299
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[Journal Article] Interindividual variation in arsenic metabolism in a Vietnamese population : association with 17 single nucleotide polymorphisms in AS3MT2010
Author(s)
Agusa, T., Iwata, H., Fujihara, J., Kunito, T., Takeshita, H., Minh, T.B., Trang, P.T.K., Viet, P.H., Tanabe, S.
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Journal Title
Interdisciplinary Studies on Environmental Chemistry-Biological Responses to Chemical Contaminants : From Molecular to Community Level (印刷中)
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[Journal Article] Contamination by trace elements in e-waste recycling sites in Bangalore, India2009
Author(s)
Ha, N.N., Agusa, T., Ramu, K., Tu, N.P.C., Murata, S., Parthasaraty, P., Bulbule, K.A., Takahashi, S., Subramanian, A., Tanabe, S.
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Journal Title
Chemosphere 76
Pages: 9-15
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[Journal Article] Zinc is an essential trace element for spermatogenesis in male2009
Author(s)
Yamaguchi, S., Miura, C., Kikuchi, K., Celino, F.T., Agusa, T., Tanabe, S., Miura, T.
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Journal Title
Proceedings of the National Academy of Sciences(PNAS) 106(26)
Pages: 10859-10864
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[Journal Article] Ethnic variation in genotype frequencies of δ-aminolevulinic acid dehydratase(ALAD)2009
Author(s)
Fujihara, J., Agusa, T., Yasuda, T., Soejima, M., Kato, H., Panduro, A., Koda, Y., Kimura-Kataoka, K., Takeshita, H.
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Journal Title
Toxicology Letters 191
Pages: 236-239
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[Journal Article] Genetic polymorphism influencing arsenic metabolism in human2009
Author(s)
Takeshita, H., Fujihara, J., Agusa, T., Takahashi, S., Iwata, H., Tanabe, S.
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Journal Title
Interdisciplinary Studies on Environmental Chemistry-Environmental Research in Asia
Pages: 191-195
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[Journal Article] Relationship of urinary arsenic metabolites to intake estimates in residents of the Red River Delta, Vietnam2009
Author(s)
Agusa, T., Kunito, T., Minh, T. B., Trang, P. T. K., Iwata, H., et al.
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Journal Title
Environmental Pollution 157(2)
Pages: 396-403
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[Journal Article] Genetic polymorphisms in AS3MT and arsenic metabolism in residents of the Red River Delta, Vietnam2009
Author(s)
Agusa, T., Iwata, H., Fujihara, J., Kunito, T., Takeshita, H., et al.
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Journal Title
Toxicology and Applied Pharmacology 236(2)
Pages: 131-141
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[Journal Article] Relationship between genetic polymorphism of arsenic (+3 oxidation state) methyltransferase (AS3MT) and profiles of urinary arsenic compounds in Vietnamese2008
Author(s)
Agusa, T., Fujihara, J., Takeshita, H., Iwata, H., Minh, T. B., et al.
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Journal Title
Biomedical Research on Trace Elements 19(3)
Pages: 265-267
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[Presentation] Association of arsenic exposure and genetic polymorphisms with arsenic metabolism in a Vietnamese population form the Red River Delta2011
Author(s)
Agusa, T., Kunito, T., Fujihara, J., Takeshita, H., Minh, T.B., Trang, P.T.K., Viet, P.H., Tanabe, S., Iwata, H.
Organizer
The Society of Toxicology 50th Annual Meeting
Place of Presentation
Washington DC, US
Year and Date
2011-03-09
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[Presentation] Human exposure to toxic metals in Pb acid battery recycling site in Dong Mai, North Vietnam2010
Author(s)
Noguchi, T., Itai, T., Hirata, S., Agusa, T., Tue, N.M., Trang, P.T.K., Viet, P.H., Takahashi, S., Tanabe, S.
Organizer
SETAC (Society of Environmental Toxicology and Chemistry) North America 31st Annual Meeting
Place of Presentation
Portland, Oregon, USA
Year and Date
2010-11-08
Related Report
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[Presentation] Soil and air particle contamination by trace elements at e-waste recycling sites in North Vietnam : Implying human health risk2010
Author(s)
Ha, N.N., Agusa, T., Horai, S.H., Itai, T., Trang, P.T.K., Takahashi, S., Viet, P.H., Tanabe, S.
Organizer
SETAC (Society of Environmental Toxicology and Chemistry) North America 31st Annual Meeting
Place of Presentation
Portland, Oregon, USA
Year and Date
2010-11-08
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[Presentation] Determination of heavy metals at formal and informal E-waste recycling sites in the Philippines2010
Author(s)
Agusa, T., Takigami, H., Fujimori, T., Eguchi, A., Bekki, K., Yoshida, A., Terazono, A., Allesteros Jr., F.C.
Organizer
The 7th NIES Workshop on E-waste
Place of Presentation
Tsukuba, Japan
Year and Date
2010-10-19
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[Presentation] Accumulation of trace elements in harp seals (Phoca groenlandica) from Pangnirtung in Baffin island, Canada2010
Author(s)
Agusa, T., Nomura, K., Kunito, T., Anan, Y., Iwata, H., Tanabe, S.
Organizer
6th International Conference on Marine Pollution and Ecotoxicology
Place of Presentation
Hong Kong, China
Year and Date
2010-06-02
Related Report
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[Presentation] Methylation of inorganic arsenic and genetic polymorphisms in arsenic (+3 oxidation state) methyltransferase in a Vietnamese population2010
Author(s)
Agusa, T., Fujihara, J., Takeshita, H., Tanabe, S., Iwata, H., Kunito, T., Minh, T.B., Trang, P.T.K., Viet, P.H.
Organizer
The Third International Congress on Arsenic in the Environment (As 2010)
Place of Presentation
Tainan, Taiwan
Year and Date
2010-05-20
Related Report
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[Presentation] Soil contamination by trace elements at e-waste recycling sites in Vietnam and India2010
Author(s)
Ha, N.N., Agusa, T., I, Tue, N.M., Minh, T.B., Minh, N.H., Itai, T., Takahashi, S., Subramanian, A., Viet, P.H., Tanabe, S.
Organizer
The First International Conference on Environmental Pollution, Restoration and Management
Place of Presentation
Ho Chi Minh City
Related Report
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[Presentation] Arsenic, manganese and barium contamination in groundwater and human in Vietnam2010
Author(s)
Agusa, T., Inoue, S., Kunito, T., Minh, T.B., Tu, N.P.C., Ha, N.N., Trang, P.T.K., Iwata, H., Tuyen, B.T., Viet, P.H., Tanabe, S.
Organizer
The First International Conference on Environmental Pollution, Restoration and Management
Place of Presentation
Ho Chi Minh City
Related Report
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[Presentation] ベトナムe-waste リサイクル処理現場におけるヒトの微量元素曝露の実態2009
Author(s)
野口貴子, 板井啓明, 宝来佐和子, Nguyen Mimh Tue, 高橋真, 阿草哲郎, Tu Binh Minh, Pham Thi Kim Trang, Pham Hung Viet, Minh Nguyen Hung, 田辺信介
Organizer
第15回日本環境毒性学会・バイオアッセイ研究会合同研究発表会
Place of Presentation
東京・品川
Related Report
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[Presentation] Interindivdiual variation in arsenic metabolism in a Vietnamese population : association with single nucleotide polymorphisms in AS3MT2009
Author(s)
阿草哲郎, 岩田久人, 藤原純子, 國頭恭, 竹下治男, Minh, T.B., Trang, P.T.K., Viet, P.H., 田辺信介
Organizer
Biological Responses to Chemical Contaminants : from molecular to community level(招待)
Place of Presentation
Aveiro, Portugal
Related Report
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[Presentation] Soil contamination by trace elements at e-waste recycling sites in Vietnam and India2009
Author(s)
Ha, N.N., Agusa, T., Tue, N.M., Minh, T.B., Minh, N.H., Itai, T., Takahashi, S., Subramanian, A., Viet, P.H., Tanabe, S.
Organizer
International Symposium on Environmental Specimen Bank -Exploring Possibility of Setting-up ESBs in Developing Countries
Place of Presentation
松山
Related Report
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[Presentation] Distribution and biomagnification of arsenic species in the deep-sea food web of Sagami Bay, Japan2009
Author(s)
Hayase, D., Toyoshima, S., Agusa, T., Takahashi, S., Horai, S., Itai, T., Omori, K., Nishida, S., Tanabe, S.
Organizer
International Symposium on Environmental Specimen Bank -Exploring Possibility of Setting-up ESBs in Developing Countries
Place of Presentation
松山
Related Report
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[Presentation] Association of inorganic arsenic metabolism with genetic polymorphisms of GST isoforms in north Vietnamese populations : utilization of stored samples in the Environmental Specimen Bank of Ehime University2009
Author(s)
阿草哲郎, 岩田久人, 藤原純子, 國頭恭, 竹下治男, Minh, T.B., Trang, P.T.K., Viet, P.H., 田辺信介
Organizer
International Symposium on Environmental Specimen Bank -Exploring Possibility of Setting-up ESBs in Developing Countries
Place of Presentation
松山
Related Report
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[Presentation] Urinary arsenic metabolites in populations exposed to arsenic in groundwater from Cambodia, India, and Vietnam2008
Author(s)
Agusa, T., Inoue, S., Kunito, T., Ramu, K., Charaborty, P., et al.
Organizer
5th SETAC (Society of Environmental Toxicology and Chemistry) World Congress
Place of Presentation
Sydney, Australia
Year and Date
2008-08-06
Related Report
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[Book] Chemical Pollution in Indochina-Contamination Status, Ecosystem Impact and Remediation Technology2009
Author(s)
Agusa, T., Iwata, H., Inoue, S., Kubota, R., Minh, T.B., Tanabe, S.
Total Pages
200
Publisher
Tokai University Press
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[Book] Chemical Pollution in Indochina2009
Author(s)
Agusa, T., Iwata, H., Minh, T. B., Tanabe, S., Inoue, S., et al.
Total Pages
200
Publisher
Tokyo University Press
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