| Project/Area Number |
09041150
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Field Research |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MIYAZAWA Takayuki (1998) the Univ.of Tokyo, Grad Sch.of Agri.And Life Sci., Assistant Professor, 大学院・農学生命科学研究科, 助手 (80282705)
見上 彪 (1997) 東京大学, 大学院・農学生命科学研究科, 教授 (20091506)
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| Co-Investigator(Kenkyū-buntansha) |
HORIMOTO Taisuke Osaka Prefecture University, Faculty of Agriculture Associate Professor, 農学部, 助教授 (00222282)
ONO Ken-ichiro the Univ.of Tokyo, Grad Sch.of Agri.And Life Sci., Professor, 大学院・農学生命科学研究科, 教授 (50111480)
DOI Kunio the Univ.of Tokyo, Grad Sch.of Agri.And Life Sci., Professor, 大学院・農学生命科学研究科, 教授 (70155612)
TAKAHASHI Eiji the Univ.of Tokyo, Grad Sch.of Agri.And Life Sci., Professor, 大学院・農学生命科学研究科, 教授 (50183439)
MIKAMI Takeshi Obihiro Univ.of Agri.and Vet.Med.Res.The Res.Center for Protozoan Mol.Immunol.,, 原虫病分子免疫研究センター, 教授 (20091506)
宮沢 孝幸 東京大学, 大学院・農学生命科学研究科, 助手 (80282705)
遠矢 幸伸 鹿児島大学, 農学部, 助教授 (30021702)
望月 雅美 共立商事(株), 臨床微生物研究所, 所長 (90157834)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥9,600,000 (Direct Cost: ¥9,600,000)
Fiscal Year 1998: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1997: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Keywords | Vietnam / feline immunodeficiency virus / feline herpesvirus type 1 / feline leukemia virus / feline svncvtial virus / feline calicivirus / feline parvovirus / leopard cat |
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| Research Abstract |
In Socialist Republic of Vietnam (Vietnam), there are lots of camivors (feuds) which are not present in Japan. Three retroviruses (feline leukemia virus (FIV), feline immunodeficiency virus (FIV), and feline syncytial virus (FSV)) are known to infect domestic cats. The purpose of this study is to isolate and identify retroviruses from several feuds in Vietnam and to compare them with known retroviruses. We surveyed domestic and leopard cats in the vicinity of Hanoi and HoChiMinh city.
In the vicinity of Hanoi, we could not find any incidence of infection of FIV or FeLV in both domestic and leopard cats. However, we found that 29% of domestic cats and 71% of leopard cats were infected with FSV.We isolated 9 strains of FSV and conducted phylogenetic analysis. All the isolates except one isolate formed a cluster in the phylogenetic tree. Form the result, we considered that FSV has been present in Vietnam from the earliest time or FIV of domestic cats infect leopard cats recently, or vice versa.
In the vicinity of HoChiMinh city, we could not find any incidence of infection of FeLV in both domestic and leopard cats. Contrary to the result obtained in the vicinity of Hanoi city, we found that 22% of domestic cats were ***ected with FIV whereas no leopard cats were infected with the virus. In addition, we found that 78% of domestic cats and 25% of leopard cats were infected with FSV.
We succeeded in isolation of five strains of FIV and determined the nucleotide sequence of the V3-V5 legion of env gene. The phylogenetic analysis revealed that five strains belonged to subtype C and one strain belonged to subtype D.FIV Subtype C is reported to be prevalent in Canada and Taiwan. From the results obtained here, we considered that FIV invaded southern Vietnam recently from other countries including Canada and Taiwan, or domestic cats in southern Vietnam retained FIV subtype C from the earliest time.
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