Grant-in-Aid for International Scientific Research.
Bacteriology (including Mycology)
|Research Institution||Chiba University|
FUKUSHIMA Kazutaka Chiba Univ.Res.Ctr.Pathogeric Fungi & Microbial Toxcoses Assoc.Prof., 真菌医学研究センター, 助教授 (90114321)
DE Lima Fith ブラジル連邦ペルナンブコ大学, 生物科学部, 助教授
DE CamposーTa ブラジル連邦ペルナンブコ大学, 生物科学部, 教授
DE Carvalho ブラジル連邦ペルナンブコ大学, 生物科学部, 教授
YOKOYAMA Koji Chiba Univ.Res.Ctr.Pathogeric Fungi & Microbial Toxcoses Res.Assoc, 助手 (80092112)
吉田 祚一 千葉大学, 真菌医学研究センター, 助手 (20114340)
亀井 克彦 千葉大学, 真菌医学研究センター, 助教授 (10214545)
NISHIMURA Kazuko Chiba Univ.Res.Ctr.Pathogeric Fungi & Microbial Toxcoses Prof., 教授 (00114314)
MIYAJI Makoto Chiba Univ.Res.Ctr.Pathogeric Fungi & Microbial Toxcoses Prof., 教授 (40009494)
GALBA Maria ブラジル連邦ペルナンブコ大学, 生物科学部, 教授
HORIE Yoshikazu Natural Musium Chiba Prefcture Curator, 科長 (40092093)
CAMPOS-TAKAKI Galba M. Pernumbuco Univ.Brazil Ctr Siencia Biologica Prof.
|Project Fiscal Year
1997 – 1998
Completed(Fiscal Year 1998)
|Budget Amount *help
¥6,500,000 (Direct Cost : ¥6,500,000)
Fiscal Year 1998 : ¥3,100,000 (Direct Cost : ¥3,100,000)
Fiscal Year 1997 : ¥3,400,000 (Direct Cost : ¥3,400,000)
|Keywords||pathogenic fungi / eology / molecular epidemiology / Brazil / gene / dematiaceous fungi / dermatophytes / 病原真菌 / 生態 / 分子疫学 / 黒色真菌 / 遺伝子 / ブラジル / 皮膚糸状菌|
In the present work, following new evidences were obtained.
1. Genetic identification method of Trichophyton tonsurans, a dermatophyte which is highly distributed in world wide as European and both American countries but low in Asia, was established. Furthermore, it was demonstrated that there is no genetically different among the strains isolated from various regions of the world.
2. To study genetic variations of three dematiaceous fungi, Cladophialophora carrioni, Fonsecaea pedrosoi and Phialophora verrucosa which are causative agents of chromoblastomycosis, random amplified polymorphic DNA analysis (RAPD) was applied to the isolates collected from vaiious areas of the world. The results showed that the strains isolated from the same country or area had genetically high homogeneous pattern differed from those of other areas.
3. The physiological and morphological features of Paracoccidioides brasiliensis, the causative agent of paracoccidioidomycosis which is a deep mycosis limited to Latin America, were studied in order to define their phenotype. RAPD analysis for the strains collected in Brazil showed that the tested strains could be separated in two groups genetically.
4. The newly designated primers from known cytochrome b amino acid sequences were found to be useful for identification and phylogenetic study of pathogenic species of Aspergillus.