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Studies on Epstein-Barr virus vector for human gene therapy

Research Project

Project/Area Number 09044246
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field Virology
Research InstitutionHokkaido University

Principal Investigator

TAKADA Kenzo  Hokkaido University school of Medicine, Professor, 医学部, 教授 (30133721)

Co-Investigator(Kenkyū-buntansha) MARUO Seiji  Hokkaido University School of Medicine・Assistant, 医学部, 助手 (70292018)
IMAI Shousuke  Hokkaido University School of Medicine・Assistant professor, 医学部, 助教授 (60232592)
サダデン ビル  ウイスコンシン大, マカードル研究所, 副所長兼教授
ハンマーシュミット ウォ  ドイツ臨床分子生物学, 腫瘍遺伝子研究所, 部長
WOLFGANG Hammerschmidt  Institute of Moleculerbiology and Tumorgenetics ・ Chief
SUGDEN Bill  McArdle Laboratory, Wisconsin University・Assistantmanager, Professor
サグデン ビル  ウィスコンシン大, マカードル研究所, 副所長兼教授
ウォルフガング ハンマー  ドイツ臨床分子生物学, 腫瘍遺伝子研究所, 部長
杉浦 亮  北海道大学, 医学部, 助手 (20241317)
ビル サグデン  ウィスコンシン大学, マカードル研究所, 副所長兼教授
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1997: ¥4,200,000 (Direct Cost: ¥4,200,000)
KeywordsEB virus / Gene therapy / Viral vector
Research Abstract

There has been no infection system such as that winch produces viruses following Epstein-Barr virus (EBV infection, Therefore, it has not been possible to produce a large amount of recombinant EBV for gene therapy. In this study, we aimed to generate an amplicon system of EBV.DNA sequences containing the replication origin in a virus replicative phase and packaging signals are efficiently amplified and packaged into virus particles during virus replication. We generated cells infected with recombinant EBV with more than 200kbp genome size. Such large EBV genomes exceed the size limitation for being packaged into virus particles. Therefore, such cells should become an ideal packaging cells for propagating amplicon virus. We confirmed that Akata cells carrying huge EBV genome produce lyric antigen but do not produce progeny viruses upon treatment with anti-lg antibodies. Base on these results, we genierated a plasmid containing the replication origin and packaging signal of EBV and GFP gene, and transfected it into the Akata cells carrying huge EBV genome. We confirmed that the transfected plasmid is packaged and produced as infectious viruses after anti-lg treatment.
Dr.Takada and Dr.lmai visited Dr.Hammerschmidt's laboratory in June, 1998. During its visit, we discussed about mutual experimental results, and obtained many important informations each other.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Koide, J.: "Spontaneous establishment of an Epstein-Barr virus-infected fibroblast line from the synovial tissue of a rheumatoid arthritis patient." J.Virol.71. 2478-2481 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wen, S.: "Epstein-Barr virus (EBV) infection in salivary gland tumors : lytic EBV inlection in nonmalignant epithelial cells surrounded by EBV-positive T-lymphoma cells." Virology. 227. 484-487 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshiyama, H.: "Epstein-Barr virus infection to human gastric carcinoma cells. implication of the existense of a new virus receptor different from CD2l." J.Virol.71(7). 5688-5691 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yanai, H.: "Endoscopic and pathologic features of Epstein-Barr virus-associated gastric carcinoma" Gastrointest. Endosco.45(3). 236-242 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yanai, H.: "Epstein-Barr virus infection in non-carcinomatous gastric epithelium" J.Pathol.183(3). 293-298 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mizugaki, Y.: "Detection of Epstein-Barr virus in oral papilloma." Jap.J.Cancer Res.89. 604-607 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Imai, S.: "Cell-to-cell contact as an efficient mode of Epstein-Barr virus infection of diverse human epithelial cells." J.Virol.72. 4371-4378 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Komano, J.: "Epstein-Barr virus contributes to the malignant phenotype and to apoptosis resistance in Burkitt's lymphoma cell line Akata." J.Virol.72. 9150-9156 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sugawara, Y.: "Detection of EBV in hepatocellular carcinoma tissue : a novel EBV latency characterized by absence of EBER expression." Virology. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Torii, T.: "The truncated form of the Epstein-Barr virus LMPI is dispensable or complimentable by the full-length form in virus infection and replication." Virology. 251. 273-278 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nishikawa, J.: "EBV promotes epithelial cell growth in the absence of EBNA2 and LMPI expression" J.Virol.73. 1286-1292 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Koide, J.: "Spontaneous establishment of an Epstein-Barr virus-infected fibroblast line from the synovial tissue of a rheumatoid arthritis patient." J.Virol.71. 2478-2481 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wen, S.: "Epstein-Barr virus (EBV) infection in salivary gland tumors : lytic EBV infection in nonmalignant epithelial cells surrounded by EBV-positive T-lymphoma cells." Virology. 227. 484-487 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshiyama, H.: "Epstein-Barr virus infection to human gastric carcinoma cells : implication of the existense of a new virus receptor different from CD21." J.Virol.71 (7). 5688-5691 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yanai, H.: "Endoscopic and pathologic features of Epstein-Barr virus-associated gastric carcinoma." Gastrointest.Endosco.45 (3). 236-242 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yanai, H.: "Epstein-Barr virus infection in non-carcinomatous gastric epithelium." J.Pathol.183 (3). 293-298 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mizugaki, Y.: "Detection of Epstein-Barr virus in oral papilloma." Jap.J.Cancer Res.89. 604-607 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Imai, S.: "Cell-to-cell contact as an efficient mode of Epstein-Barr virus infection of diverse huma epithelial cells." J.Virol.72. 4371-4378 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Komano, J.: "Epstein-Barr virus contributes to the malignant phenotypeand to apoptosis resistance in Burkitt's lymphoma cell line Akata." J.Virol.72. 9150-9156 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sugawara, Y.: "Detection of EBV in hepatocellular carcinoma tissue : a novel EBV latency characterized by absence of EBER expression." (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Torii, T.: "The truncated from of the Epstein-Barr virus LMP1 is dispensable or complimentable by the full-length form in virus infection andreplication." Virology. 251. 273-278 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nishikawa, J.: "EBV promotes epithelial cell growth in the absence of EBNA2 and LMP1 expression." J.Virol.73. 1286-1292 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nishikawa,J.: "EBV promotes epithelial cell growth in the absence of EBNA2 and LMP1 expression." J.Virol.73. 1286-1292 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Imai,S.: "Cell-to-cell contact as an efficient mode of Epstein-Barr virus infection of diverse human epithelial cells." J.Virol.72. 4371-4378 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Komano,J.: "Epstein-Barr virus contributes to the malignant phenotype and to apoptosis resistance in Burkitt's lymphoma cell line Akata." J.Virol.72. 9150-9156 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sugawara,Y.: "Detection of EBV in hepatocellular carcinoma tissue:a novel EBV latency characterized by absence of EBER expression." Virology. (in press)

    • Related Report
      1998 Annual Research Report
  • [Publications] Torii,T.: "The truncated form of the Epstein-Barr virus LMP1 is dispensable or complimentable by the full-length form in virus infection and replication." Virology. 251. 273-278 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mizugaki,Y.: "Detection of Epstein-Barr virus in oral papilloma." Jap.J.Cancer Res.89. 604-607 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Koide, J.: "Spontaneous establishment of an Epstein-Barr virus-infected fibroblast line from the synovialtissue of a rheumatoid arthritis patient" J.Virol.71. 2478-2481 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Wen, S.: "Epstein-Barr virus (EBV) infection in salivary gland,tumors : lytic EBV infection in nonmalignant epithelial cells surrounded by EBV-positive T-lymphoma cells" Virology. 227. 484-487 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshiyama, H.: "Epstein-Barr virusinfection to human gastric carcinoma cells : implication of the existense of a new virus receptor different from CD21" J.Virol.71(7). 5688-5691 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yanai, H.: "Endoscopic and pathologic features of Epstein-Barr virus-associated gastric carcinoma" Gastrointest.Endosco.45(3). 236-242 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yanai, H.: ":Epstein-Barr virus-infection in non-carcinomatous gastric epithelium" J.Pathol.183(3). 293-298 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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