Grant-in-Aid for Scientific Research (B).
|Allocation Type||Single-year Grants|
Applied veterinary science
|Research Institution||TOTTORI UNIVERSITY|
MINAMI Saburo TOTTORI UNIVERSITY, AGRICULTURE, ASSOCIATE PROFESSOR, 農学部, 教授 (70032307)
SAIMOTO Hiroyuki TOTTORI UNIVERSITY, ENGINEERING, ASSOCIATE PROFESSOR, 工学部, 助教授 (20186977)
SHIGEMASA Yoshihiro TOTTORI UNIVERSITY, ENGINEERING, PROFESSOR, 工学部, 教授 (00032029)
OKAMOTO Yoshiharu TOTTORI UNIVERSITY, AGRICULTURE, ASSOCIATE PROFESSOR, 農学部, 助教授 (50194410)
TANAKA Yoshinori TOTTORI UNIVERSITY, MEDICINE, PROFESSOR, 医学部, 教授 (70029809)
SASHIWA Hitoshi TOTTORI UNIVERSITY, ENGINEERING, ASSOCIATE PROFESSOR, 工学部, 助教授 (20205884)
作野 友康 鳥取大学, 農学部, 教授 (10032567)
山崎 良平 鳥取大学, 農学部, 教授 (80273887)
NORRDIN RW コロラド州立大学, 獣医学部, 教授
STASHAK Ts コロラド州立大学, 獣医学部, 教授
NELSON AW コロラド州立大学, 獣医学部, 教授
STASHAK TS コロラド州立大学, 獣医学部, 教授
|Project Period (FY)
1997 – 1999
Completed(Fiscal Year 1999)
|Budget Amount *help
¥3,200,000 (Direct Cost : ¥3,200,000)
Fiscal Year 1999 : ¥1,600,000 (Direct Cost : ¥1,600,000)
Fiscal Year 1998 : ¥800,000 (Direct Cost : ¥800,000)
Fiscal Year 1997 : ¥800,000 (Direct Cost : ¥800,000)
|Keywords||AMINO SUGAR / CHITIN / CHITOSAN / BIOLOGICAL DEFENSE / COMPLEMENT ACTIVATION / POLYMORPHONUCLEAR CELLS / CHEMILUMINESCENCE / GRANULATING TISSUE FORMATION|
1)Development of medical products from chitin : Water soluble chitins were developed. Chitin is a highly biocompatible material, but is not water-soluble , and it is soluble only in formic acid. The water-soluble chitins were produced by chemical modification of chitin such as carboxymetylation, sulfation, and phosphorylation of chitin (Shigemasa, Saimoto and Sashiwa).
2)Veterinary clinical application of the water-soluble chitins : These chitins were tested for a blocker of murine experimental pneumonia induced by chitosan. After intraperitoneal administration of chitosan to mice, these chitins were administered intravenously. Various degree of blocking effects by these chitins was observed, however, the phosphorylation one was most effective (Minami, Okamoto). After depolymerization of the phosphorylation chitin, the blocking effect of it was decreasing.
3)A mechanism of pneumonia blocking effect of the water-soluble chitin : We already investigated that chitosan was a chemotactic agent, and a stimulator of chemiluminescence of polymorphonuclear cells. Further more, this effect was brought by activation of complement system via the alternative pathway, and produced C5a was a trigger substance for the excitement of polymorphonuclear cells (Minami, Okamoto). To prevent the pneumonia by chitosan, it will need to block complement activation of inflammatory cells by the soluble-chitin. We investigated the complement blocking effect of the soluble-chitins by single radial immunodiffusion for C3 activation, however, all chitins did not block the C3 activation. We speculate that these soluble-chitins will prevent the migration of inflammatory cells in mice.