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Metabolic evaluation using in vivo NMR spectroscopy for individual organs under serious conditions associated with reductive liver mitochondrial state

Research Project

Project/Area Number 09307024
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionSHIGA UNIVERSITY OF MEDICAL SCIENCE

Principal Investigator

OZAWA Kazue  SHIGA UNIV. MED. SCI., DEPT. OF MEDICINE, PRESIDENT, 医学部, 学長 (00026858)

Co-Investigator(Kenkyū-buntansha) MORIKAWA Shigehiro  SHIGA UNIV. MED. SCI., MOL. NEUROBIOL. RES. CTR. ASSOCIATE PROFESSOR, 分子神経科学研究センター, 助教授 (60220042)
TABATA Ryoko  SHIGA UNIV. MED. SCI., DEPT. OF MEDICINE, PRESIDENT, 医学部, 教授 (20155229)
INUBUSHI Toshihiro  SHIGA UNIV. MED. SCI., MOL. NEUROSCI. RES. CTR. PROFESSOR, 分子神経科学研究センター, 教授 (20213142)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥29,500,000 (Direct Cost: ¥29,500,000)
Fiscal Year 1999: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1998: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1997: ¥21,300,000 (Direct Cost: ¥21,300,000)
KeywordsAKBR / liver mitochondria / systemic care / in vivo NMR spectroscopy / energy metabolism / アポトーシス / ^<31>P NMR / ^<13>C NMR
Research Abstract

Even in severe circulatory insufficient conditions during hemorrhage in jaundiced rats, the phosphoenergetic level in the myocardium was maintained fairly well. In such conditions, arterial ketone body ratio (AKBR) decreased and it was found to be a potential parameter to evaluate the systemic metabolic state. We also applied ィイD131ィエD1P- and ィイD113ィエD1C-NMR spectroscopy together to examine the metabolic state of the liver. Furthermore, the metabolic states of individual organs, such as liver, heart, brain and skeletal muscles, were investigated with NMR spectroscopy using hemorrhagic shock, endotoxic shock, myocardial infarction, brain infarction, epilepsy and diabetic animal models. We incorporated ultra-fast echo planar imaging (EPI) technique into ィイD11ィエD1H-NMR spectroscopic imaging. We have already reported fast ィイD113ィエD1C metabolite mapping derived from ィイD113ィエD1C-glucose in rat focal brain ischemia.
These NMR spectroscopic techniques were also applied to the clinical cases. In patients with diabetic gangrene, we found metabolic abnormalities in the skeletal muscles associated with increased pII. Such findings were clearly different from that with ischemia. For heart disease, ィイD131ィエD1P NMR spectroscopy during hand-grip exercise was found to be useful to judge the viability of myocardium. In brain research ィイD11ィエD1H NMR spectroscopy was carried out for the judgment of malignant potential of maningioma, the pathophysiologic condition of myotonic dystrophy, spectroscopy during photic stimulation was used for the prediction of the effects of lithium or electrical stimulation therapy for bipolar disorder. Fast NMR spectroscopic imaging technique could be also applicable with clinical cases.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] Morikawa, S et al.: "Gluconeogenesis and phosphoenergetics in rat liver during endotoxemia"J. Surg. Res.. 74. 179-186 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takahashi, K et al.: "Hepatic energy metabolism during ketamine and iscflurane anesthesia in haemorrhagic shock"Br. J. Anaesth.. 80. 782-787 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Morikawa, S et al.: "Effects of blood suger level or rat transient focl brain ischemia consecutively observed by…"Magn. Reson. Med.. 42. 895-902 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Katsuyama, K et al.: "Myocardial high-energy phosphate and hepatic redox state in jaundiced rats"J. Surg. Res.. 82. 88-94 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takaoka A et al.: "Renal ischemia/reperfusion remotely imporves myocardial energy metabolism during myocardial…"J. Am. Coll. Cardiol.. 33. 556-564 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shiino A et al.: "Noninvasive evaluation of the malignant potential of intracranial meningiomas performed using…"J. Neurosurg.. 91. 928-934 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Morikawa S, et al.: "Gluconeogenesis and phosphonergetics in rat liver during endotoxemia"J. Surg. Res.. 74. 179-186 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Terasaki M, et al.: "Effects of Transjugular Intrahepatic Portosystemic Shunts on Hepatic Metabolic Function Determined with Serial Monitoring of Arterial Ketone Bodies"JVIR. 9. 129-135 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishii H, et al.: "Evaluation of focal cerebral ischemia in rats by magnetic resonance imaging and immunohistochemical analyses"J. Cereb.. Blood Metab. 18. 934-934 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishida T, et al.: "A primitive pathway of porphyrin biosynthesis and enzymology in Desulfovibrio vulgaris"Proc. Natl. Acad. Sci.. USA 95. 4853-4858 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Okada M, et al.: "Influence of aging or left ventricular hypertrophy on the human heart : contents of phosphorus metabolites measured by ィイD131ィエD1P MRS"Magn Reson. Med.. 38. 772-782 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakajima M, et al.: "Estimation of volume doubling time and cell loss in an experimental rat glioma model in vivo"Acta Neurochir (Wien). 140. 607-613 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takahashi, K., et al.: "Hepatic energy metabolism during ketamine and isoflurane anesthesia in haemorrhagic shock"Br. J. Anaesth.. 80. 782-787 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kato, T., et al.: "Photic stimulation-induced alteration of brain energy metabolism measured by ィイD131ィエD1P MR spectroscopy in patients with MELAS"J Neurol Sci.. 155. 182-185 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Morikawa S, et al.: "Effects of blood sugar level on rat transient focal brain ischemia consecutively observed by diffusion-weighted EPI and 1H echo planar spectroscopic imaging"Magn Reson Med. 42. 895-902 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Katsumiya K, et al.: "Myocardial high-energy phosphates and hepatic redox state in jaundiced rats"J. Surg. Res.. 82. 88-94 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takaoka A, et al.: "Renal ischemia/reperfusion remotely improves myocardial energy metabolism during myocardial ischemia via adenosine receptors in rabbits : effects of "remote preconditioning""J. Am. Coll. Cardiol.. 33. 556-564 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Murashita J, et al.: "Age-dependent alteration of metabolic response to photic stimulation in the human brain measured by 31P MR-spectroscopy"Brain Res.. 818. 72-76 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Akiguchi I, et al.: "Brain proton magnetic resonance spectroscopy and brain atrophy in myotonic dystrophy"Arch. Neurol.. 56. 325-330 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shiino A, et al.: "Noninvasive evaluation of the malignant potential of intracranial meningiomas performed using proton magnetic resonance spectroscopy"J. Neurosurg.. 91. 928-934 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kato T, et al.: "Relationship of energy metabolism detected by ィイD131ィエD1P-MRS in the human brain with mental fatigue"Neuropsychobiology. 39. 214-218 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Morikawa S, et al.: "Diffusion-weighted EPI ィイD11ィエD1H Echo Planar spectroscopic Imaging Studies on Rat Transient Middle Cerebral Artery Occlusion Model"Ultrafast Magnetic Resonance Imaging in Medicine, Elsevier. 209-212 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hamakawa H, et al.: "Quantitative proton magnetic resonance spectroscopy of the bilateral frontal lobes in patients with bipolar disorder"Psychol Med.. 29. 639-644 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki E, et al.: "ィイD11ィエD1H- and ィイD131ィエD1P-magnetic resonance spectroscopy and imaging as a new diagnostic tool to evaluate neuropathic foot ulcers in type 2 diabetic patients"Diabetologia. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Morikawa S. et al.: "Gluconeogenesis and phosphoenergetics in rat liver during endotoxemia"J.Surg.Res.. 74. 179-186 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takahashi K. et al.: "Hepatic energy metabolism during ketamine and isoflurane anaesthesia in haemorrhagic shock"Br.J.Anaesth.. 80. 782-787 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Morikawa S. et al.: "Effects of blood sugar level on rat transient focal brain ischemia consecutively observed by・・・・・・・"Magn.Reson.Med.. 42. 895-902 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Katsuyama K. et al.: "Myocardial high-energy phosphates and hepatic redox state in jaundiced rats"J.Surg.Res.. 82. 88-94 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takaoka A. et al.: "Renal ischemia/reperfusion remotely improves myocardial energy metabolism during myocardial ischemia via・・・・"J.Am.Coll.Cardiol.. 33. 556-564 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shiino A. et al.: "Noninvasive evaluation of the malignant potential of intracranial meningiomas performed using proton・・・・・"J.Neurosurg.. 91. 928-934 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Morikawa,S.et al.: "Gluconeogenesis and phosphoenergetics in rat liver during endotoxemia." Journal of Surgical Research. 74. 179-186 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Okada,M.et al.: "Influence of aging or left ventricular hypertrophy on the human heart: contents of phosphorus metabolites measured by 31P MRS." Magnetic Resonance in Medicine. 38. 772-782 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takahashi,K.et al.: "Hepatic energy metabolism during ketamine and isoflurane anaesthesia in haemorrhagic shock." British Journal of Anaesthesia. 80. 782-787 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ishi,H.et al.: "Evaluation of focal cerebral ischemia in rats by magnetic resonance imaging and immunohistochemical analyses." Journal of Cerebral Blood Flow and Metabolism. 18. 931-934 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takaoka,A.et al.: "Renal ischemia/reperfusion remotely improves myocardial energy metabolism during myocardial ischemia via adenosine receptors." Journal of American College of Cardiology. 33. 556-564 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Katsuyama,K.et al.: "Myocardial high energy phosphates and hepatic redox state in jaundiced rats." Journal of Surgical Research. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Morikawa S, et al.: "Relationship between gluconeogenesis,and phosphoenergetics in rat liver assessed by in vivo 13C and 31P NMR spectroscopy." NMR Biomed. 10. 18-24 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takahashi K, et al.: "Effects of halothane and isoflurane on phosphoenergetic state of the liver during hemorrhagic shock in rats." Anesth Analg. 85. 347-352 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Morikawa S, et al.: "Gluconeogenesis and phosphoenergetics in rat liver during endotoxemia." J Surg Res. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Ishii H, et al.: "Evaluation of focal cerebral ischemia in rats by magnetic resonance imaging and immunohistochemical analyses." J Cereb Blood Flow Metab. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Takahashi K, et al.: "Hepatic energy metabolism during ketamine and isoflurane anesthesia in haemorrhagic shock." Br J Anesth. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Okada M, et al.: "Influence of aging or left ventricular hypertrophy on the human heart : contents of phosphorus metabolites measured by 31P MRS." Magn Reson Med. (in press).

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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