Characterization of genes involved in regulation of differentiation using mutant plant cell cultured lines by insertion of transposable elements
| Project/Area Number |
09440264
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
OZEKI Yoshihiro Tokyo University of Agriculture and Technology, Faculty of Technology, Associate Professor, 工学部, 助教授 (50185592)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1997: ¥11,500,000 (Direct Cost: ¥11,500,000)
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| Keywords | Daucus carota / plant cell culture / culture variation / transposable element / secondary metabolism / anthocyanin |
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| Research Abstract |
An En/Spm transposable elements of Tdc1 was found in the promoter region of phenylalanine ammonia-lyase gene in carrot suspension cultured cells. Tdc1 had only one long open reading frame corresponding to one transposase, but the other transposase, which is necessary for excision and insertion of Tdc1, was partially encoded in Tdc1, suggesting that Tdc1 should be a non-autonomous transposable element Southern analysis indicates that more than 20 copies of Tdc1-like elements may be in the carrot genome. The other elements of Tdc family were obtained using PCR and the nucleotide sequences were analyzed. Three sub-family of Tdc, Tdc-A, Tdc-B and TdcR, were found in carrot genome. Tdc-A1 encoded two transposases which were partially similar to maize En/Spm transposable element, suggesting that Tdc-A1 might be the autonomous element of Tdc family. The other Variants of Tdc-A showed the internal deletions without changes in nucleotide sequences. These results suggest that Tdc-A actively transposed accompanied with deletion during subculturing of carrot cells, which results in culture variation. Tdc-B and Tdc-R were deletion variants of Tdc-A.Their nucleotide sequences were changed in comparison with Tdc-A.They might transpose before the establishment of carrot cell culture.
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Report
(3 results)
Research Products
(8 results)
