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Molecular mechanisms of gametophytic self-incompatibility in Prunus species

Research Project

Project/Area Number 09460018
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field 園芸・造園学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

TAO Ryutaro  Kyoto University, Graduate School of Agncalture, Lecturer, 農学研究科, 講師 (10211997)

Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥9,300,000 (Direct Cost: ¥9,300,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1998: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1997: ¥4,600,000 (Direct Cost: ¥4,600,000)
KeywordsS-RNase / sweet cherry / Japanese apricot / Japanese plum / almond / ア-モンド
Research Abstract

Prunus, one of the genera in the Rosaceae, includes important commercial fruit and nut tress species. Most of the Prunus species exhibit the monofactorial gametophytic self-incompatibility system which is controlled by a single locus, the S-locus, with multiple alleles. As Prunus species are unable to bear fruit parthenocarpically, they will not set fruit unless pollinated with cultivars with different S-genotypes. Thus, determination of correct pollen incompatibility groups and assignment of cultivars to the groups are essential for good crop production.
This study identifies S-RNases associated with self-incompatibility in sweet cherry, almond, Japanese plum, and Japanese apricot. cDNAs encoding the S-RNases have been cloned and DNA-sequenced, which leads to the development of a molecular typing system for S-alleles in these fruit species. Furthermore, S-RNase allele that can be used as a molecular marker for self-compatibility in Japanese apricot has been explored.

Report

(5 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Tao,R. et al.: "Identification of stylar RNases associated with gametophytic self incompatibility in almond (Prunus dulcis)"Plant Cell Physiology. 38. 304-311 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tao,R. et al.: "Molecular typing of S alleles through identification, characterization and cDNA cloning for S-RNases in sweet cherry"J.Amer.Soc.Hort.Sci.. 124. 224-233 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ymane,H.,Tao,R. et al.: "Identification and cDNA cloning for S-RNases in self-incompatible Japanese plum (Prunus salicina Lindl. cv. Sordum)"Plant Biotechnology. 16. 389-396 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamane,H.,Tao,R. et al.: "Determining S-genotypes of two sweet cherry (Prunus avium L.) cultivars,'Takasago (Rockport Bigarreau)' and 'Hinode (Early Purple)'"J.Japan.Soc.Hort.Sci.. 69. 29-34 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamane,H.,R.Tao, et al.: "Determining the S-genotypes of several sweet cherry cultivars based on PCR-RFLP analysis"J.Horticultural Sci.& Biotech.. 75. 562-567 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tao,R. et al.: "Molecular marker for self-compatibility in Japanese spricot (Prunus mume)"HortScience. 35. 1121-1123 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tao, R.et al.: "Identification of stylar RNase associated with gametophytic self-incompatibility in almond (Prunus dulcis)"Plant Cell Physiology. 38. 304-311 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tao, R.et al.: "Molecular typing of S-alleles through identification, characterization and cDNA cloning for S-RNases in sweet cherry"J.Amer.Soc.Hort.Sci.. 124. 224-233 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamane, H., R.Tao, et al.: "Identification and cDNA cloning for S-RNases in self-incompatible Japanese plum (Prunus salicina Lindl.cv.Sordum)"Plant Biotechnology. 16. 389-396 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamane, H., R.Tao, et al.: "Determining S-genotypes of two sweet cherry (Prunus avium L.) cultivars, 'Takasa--go (Rockport Bigarreau)' and 'Hinode (Early Purple)'"J.Japan.Soc.Hort.Sci.. 69. 29-34 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamane.H., R.Tao, et al.: "Determining the S-genotypes of several sweet cherry cultivars based on PCR-RFLP analysis"J.Horticultural Sci.& Biotech.. 75. 562-567 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tao, R., et al.: "Molecular marker for self-compatibility in Japanese apricot (Prunus mume)"HortScience. 35. 1121-1123 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tamura,M.,K.Ushijima,H.Sassa,H.Hirano,R.Tao,et al.: "Identification of self-incompatibility genotypes of almond by allele-specific PCR analysis"Theoretical Applied Genetics. 101. 344-349 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yamane,H.,R.Tao,H.Murayama,and A.Sugiura: "Determining the S-genotypes of several sweet cherry cultivars based on PCR-RFLP analysis"Journal of Horticultural Science & Biotechnology. 75. 562-567 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tao,R.,T.Habu,H.Yamane,A.Sugiura,and K.Iwamoto: "Molecular markers for self-compatibility in Japanese apricot (Prunus mume)"HortScience. 35. 1121-1123 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 田尾 龍太郎: "Molecular typing of S-alleles through identification characterization and cDHA cloning for S-Rnases in sweet cherry"Journal of American Society for Horticultural Science. 124・3. 224-233 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 山根 久代: "Identification and cDNA clonling for S-Rnases in self-incompatible Japanese plum (Prunus salicina Lindl cv Sordur)"Plant Biotechnology. 16・5. 389-396 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 山根 久代: "Determining S-genotypes of two sweet cherry (Prunus ariu-L.) cultivers 'Takasago (Rockport Bigarrean)' and 'Hinode (Early Purple)'"Journal of Japanese Society for Horticultural Science. 69・1. 29-34 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 田尾 龍太郎: "Cloning and nucleotide sequences of cDNAs encoding S1-, S3-, S4-, and S6-Rnases from sweet cherry"(PGR99-121) Plant Physiology. 120. 1207-1207 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 田尾 龍太郎: "Cloning of genomic DNA sequences encoding S1-, S3-, S4-, and S6-Rnases from sweet cherry"(PGR99-166) Plant Physiology. 121. 1057-1057 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 田尾 龍太郎: "Molecular markers for self-compatibility in Japanese apricot (Prunus mume)"Hort Science. (印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Ushijima, H.Sassa, R.TAO, H.Yamane, A.M.Dandeker, T.M.Grodae: "Cloning and Characterization of cDNAs encoding S-RNases From almond (Prunus dulcis)" Mol.Gen.Genet. 260. 261-268 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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