| Budget Amount *help |
¥11,600,000 (Direct Cost: ¥11,600,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1998: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1997: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Research Abstract |
RESRCH ON THE CONSERVATION OF AVIAN WAS CONDUCTED.
AT PRESENT, AVIAN GENETIC RESOURCES ARE CONSERVED AS FLOCK SINCE IT IS NOT POSSILBLE TO CONSERVE FERTILIZED EMBRYOS FOR MORE THAN 2-3 WEEKS WITHOUT IMPAIRING HATCHABILITY.NEW STRATEGY TO CONSERVE AVIAN GENETIC RESOURCES NEEDS TO BE DEVELOPED.TO ACCHIEVE THE GOAL, GERM-LLINE CHICMERAS WERE PRODUCED BY TRANSFERRING PRIMORDIAL GERM CELLS (PGC).
REDULTS OBTAINED IN THE PRESENT RESEARCH PROJECT WAS AS FOLLOWS : 1) NUMBER OF THE CIRCULATING PGC WAS HIGHEST AT STAGE 14 AND DECREASED THEREAFTER.2) TOTAL NUMBER OF CIRCULATINF PGC WAS ESTIMATED TO BE 807 AND 857 AT STAGE 14 AND 15, RESPECTIVELY.3) THE REASON FOR THE CONSIDERABLE FULCTUATION OF THE PCG CONCENTRATION IN EARCLY CHICK EMBRYO WAS PRIMARIRILY ATTRIBUTED TO THE FEMALE THAT PRODUCE EGG.4) GERM-LINE CHIMERAS WERE PRIDUCED BY TRANSFEERING CRYOPRESERVED GONADAL PGC (GPGC) COLLECTED FROM 5-DAY-OLS CHICK EMBRYO.5) NEW METHOD OF COLLECTING COCULATING PGC USING FILTRATION METHOD WAS DEVELOPED.
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