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IDENTIFICATION AND CHARACTERIZATION OF Syk FAMIRY PROTEIN TYROSINE KINASE

Research Project

Project/Area Number 09470043
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionKOBE UNIVERSITY

Principal Investigator

YAMAMURA Hirohei  KOBE UNIV., MED., PROF., 医学部, 教授 (90030882)

Co-Investigator(Kenkyū-buntansha) TAKANO Tomoko  KOBE UNIV., MED., ASSISTANT, 医学部, 助手 (30226807)
SADA Kiyonao  KOBE UNIV., MED., ASSISTANT, 医学部, 助手 (10273765)
YANAGI Shigeru  KOBE UNIV., MED., ASSISTANT, 医学部, 助手 (60252003)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 1998: ¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1997: ¥8,700,000 (Direct Cost: ¥8,700,000)
KeywordsProtein-tyrosine / Syk / vascular endothelial cells / smooth muscle cells / neurons / tyrosine phosphorylated / non-hematopoietic cells / 血管 / 平滑筋細胞 / ZAP-70 / 神経成長円錐
Research Abstract

Protein-tyrosine kinase Syk has been implicated in a variety of hematopoietic cell responses including immunoreceptor and integrin signaling. However, so far, there is no evidence of the expression and function of Syk in the non-hematopoietic tissues. in this study, we have purified 72kDa cytoplasmic protein-tyrosine kinase, having cross-reactivity with anti-Syk antibody, as a major cytosolic protein-tyrosine kinase from rat brain and blood cells-depleted rat liver. Partial sequence analysis of purified p72 kDa protein-derived peptides revealed that this protein is identical with Syk protein-tyrosine kinase. Immunohistochemical and RT-PCR northern analysis further demonstrated that Syk is expressed widely distributed in non-hematopoietic cells including vascular endothelial cells, smooth muscle cells, fibroblasts and neurons. Furthermore, Syk was significantly activated and tyrosine phosphorylated in response to a variety of ligands in non-hematopoietic cells. Thus, our results suggest the ubiquitous role of Syk in the signaling transduction of non-hematopoietic cells. We are currently attempting to establish several primary cultured cell lines derived from Syk-deficient mice. The genetic analysis of this mutant will provide a major tool in the physiological investigation of the functions of Syk. In addition to these results we studied about Syk from hematopoietic cells. The results obtained using hematopietic cells are quite useful to analyze the function of Syk in non-hematopoietic cells.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (51 results)

All Other

All Publications (51 results)

  • [Publications] Oishi,I.: "A novel drosophila receptor tyrosine kinase expressed specifically in the nervous system." J.Biol.Chem.272(18). 11916-11923 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Qin,S.: "Up-regulation of Syk activity during HL60 cell differentiation into granulocyte but not into monocyte / macrophage-lineage." Biochem.Biophys.Res.Commun.236・3. 697-701 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Qin,S.: "Distinctive functions of Syk and Lyn in mediating osmotic stress and ultraviolet C irradiation-induced apoptosis in chicken B cells." J.Biol.Chem.272(29). 17994-17999 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Rezaul,K.: "Platelet-activating factor stimulates calcium-dependent activation of protein-tyrosine kinase Syk in human B lymphocytes." Biochem.Biophys.Res.Commun.239(1). 23-27 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sada,K.: "Relocation of Syk protein-tyrosine kinase to the actin filament network and subsequent association with Fak." Eur.J.Biochem.248. 827-833 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tubokawa,M.: "Interleukin-3 activates Syk in a human myeloblastic leukemia cell line,AML193." Eur.J.Biochem.249. 792-796 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Qin,S.: "Differential regulation of oxidative and osmotic stress-induced Syk activation by both autophosphorylation and SH2 domains." Biochemistry. 37. 5481-5486 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Oishi,I: "A novel drosophila nuclear protein serine/threonin kinase expressed in the germ line during its establishment." Mech.Dev.71. 49-63 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoneya,H.: "Antibodies directed against ZAP-70 cross react with a 66kDa tyrosine kinase in the rat brain." Biochem.Biophys.Res.Commun.245(1). 140-143 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Rezaul,K.: "Involvement of reactive oxygen intermediates in lectin-induced protein-tyrosine phosphorylation of Syk in THP-1 cells." Biochem.Biophys.Res.Commun.246(3). 863-867 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Liu,Z-J.: "A critical role for cyclin C in promotion of the hematopoietic cell cycle by cooperation with c-Myc." Molecular and Cellular Biology. 18(6). 3445-3454 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Qin,S.: "A deficiency in Syk enhances ceramide-induced apoptosis in DT40 lymphoma B cells." FEBS Lett.427. 139-143 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tohyama,Y.: "Outside-in signaling of soluble and solid-phase fibrinogen throuogeh integrin αIIBβ3 is different and cooperative with each other in a megakaryoblastic leukemia cell,CMK." Blood. 92(4). 1277-1288 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Liu,Z-J.: "Functional cooperation of cyclin C and c-Mys in mediating homotypic cell,adhesion via very late antigen-4 activation and vascular cell adhesion, molecule-1 induction." Blood. 92(12). 1-13 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hitomi,T.: "Cross-iinking of the B cell receptor induces activation of phospholipase D through Syk,Btk and phospholipase C-γ2." FEBS Lett.in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sada,K.: "Effect of lectins on protein kinase activity.In Lectin Methods and Protocols." Edited by J.M.Rhodes and J.D.Milton.Humana Press Inc., 423-432 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Minami,Y.: "Roles of Syk protein tyrosine kinase in stress-induced signaling in B cells. Redox Regulation of Cell Signaling and its Clinical Applications." Marcel Dekker Inc.NY,USA(in press), (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Oishi, I.: "A novel drosophila receptor tyrosine kinase expressed specifically in the nervous system." J.Biol.Chem.272 (18). 11916-11923 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Qin, S.: "Up-regulation of Syk activity during HL60 cell differentiation into granulocyte but not into monocyte/macrophage-lineage." Biochem.Biophys.Res.Commun.236 (3). 697-701 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Qin, S.: "Distinctive functions of Syk and Lyn in mediating osmotic stress and ultraviolet C irradiation-induced apoptosis in chicken B cells." J.Biol.Chem.272 (29). 17994-17999 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Rezaul, K.: "Platelet-activating factor stimulates calcium-dependent activation of protein-tyrosine kinase Syk in human B lymphocytes." Biochem.Biophys.Res.Commun.239 (1). 23-27 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sada, K.: "Relocation of Syk protein-tyrosine kinase to the actin filament network and subsequent association with Fak." Eur.J.Biochem.248. 827-833 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tubokawa, M.: "Interleukin-3 activates Syk in a human myeloblastic leukemia cell line, AML193." Eur.J.Biochem.249. 792-796 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sada, K.: "Effect of lectins on protein kinase activity." In Lectin Methods and Protocols. Edited by J.M.Rhodes and J.D.Milton. Humana Press Inc.423-432 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Qin, S.: "Differential regulation of oxidative and osmotic stress-induced Syk activation by both autophosphorylation and SH2 domains." Biochemistry. 37. 5481-5486 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Oishi, I.: "A novel drosophila nuclear protein serine/threonin kinase expressed in the germ line during its establishment." Mech.Dev.71. 49-63 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoneya, H.: "Antibodies directed against ZAP-70 cross react with a 66 kDa tyrosine kinase in the rat brain." Biochem.Biophys.Res.Commun.245 (1). 140-143 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Rezaul, K.: "Involvement of reactive oxygen intermediates in lectin-induced protein-tyrosine phosphorylation of Syk in THP-1 cells." Biochem.Biophys.Res.Commun.246 (3). 863-867 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Liu, Z-J.: "A critical role for cyclin C in promotion of the hematopoietic cell cycle by cooperation with c-Myc." Molecular and Cellular Biology. 18 (6). 3445-3454 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Qin, S.: "A deficiency in Syk enhances ceramide-induced apoptosis in DT40 lymphoma B cells." FEBS Lett.427. 139-143 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Minami, Y.: "Roles of Syk protein tyrosine kinase in stress-induced signaling in B cells." Redox Regulation of Cell Signaling and its Clinical Applications. Marcel Dekker Inc.NY,USA. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tohyama, Y.: "Outside-in signaling of soluble and solid-phase fibrinogen through integrin alphaIIBbeta3 is different and cooperative with each other in a megakaryoblastic leukemia cell, CMK." Blood. 92 (4). 1277-1288 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Liu, Z-J.: "Functional cooperation of cyclin C and c-Myc in mediating homotypic cell, adhesion via very late antigen-4 activation and vascular cell adhesion, molecule-1 induction." Blood. 92 (12). 1-13 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hitomi, T.: "Cross-iinking of the B cell receptor induces activation of phospholipase D through Syk, Btk and phospholipase C-gamma2." FEBS Lett.(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Qin,S.: "Differential regulation of oxidative and osmotic stress-induced Syk activation by both autophosphorylation and SH2 domains." Biochemistry. 37. 5481-5486 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Oishi,I.: "A novel drosophila nuclear protein serine/threonin kinase expressed in the germ line during its establishment." Mech.Dev.71. 49-63 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoneya,H.: "Antibodies directed against ZAP-70 cross react with a 66 kDa tyrosine kinase in the rat brain." Biochem.Biophys.Res.Commun.245(1). 140-143 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Rezaul,K.: "Involvement of reactive oxygen intermediates in lectin-induced protein-tyrosine phosphorylation of Syk in THP-1 cells." Biochem.Biophys.Res.Commun.246(3). 863-867 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Liu,Z-J.: "A critical role for cyclin C in promotion of the hematopoietic cell cycle by cooperation with c-Myc." Molecular and Cellular Biology. 18(6). 3445-3454 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Qin,S.: "A deficiency in Syk enhances ceramide-induced apoptosis in DT40 lymphoma B cells." FEBS Lett.427. 139-143 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tohyama,Y.: "Outside-in signaling of soluble and solid-phase fibrinogen through integrin α IIB β3 is different and cooperative with cach pther in a megakaryoblastic leukemia cell,CMK." Blood. 92(4). 1277-1288 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Liu,Z-J.: "Functional cooperation of cyclin C and c-Mycin mediating homotypic cell,adhesion via very late antigen-4 activation and vascular cell adhesion,molccule-1 induction." Blood. 92(12). 1-13 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hitomi,T.: "Cross-iinking of the B cell receptor induces activation of phospholipase D through Syk,Btk and phospholipase C-γ2." FEBS Lett.'(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sada,K.: "Effect of lectins on protein kinase activity. In Lectin Methods and Protocols." Edited by J.M.Rhodes and J.D.Milton. Humana Press Inc., 423-432 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Minami,Y.: "Roles of Syk protein tyrosine kinase in stress-inkuced signaling in B cells. Redox Regulation of Cell Signaling and its Clinical Applications." Marcel DekkerInc.NY,USA.(in press), (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Qin,S. et. al.: "Up-regulation of Syk activity during HL60 cell differentiation into granulocyte but not into monocyte/macrophage-lineage." Biochem. Biophys. Res. Commun.236 (3). 697-701 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Qin,S. et. al.: "Distinctive Functions of Syk of and Lyn in Mediating Osmotic Stress and Ultraviolet C Irradiation-induced Apoptosis in Chicken B cells." J. Biol. Chem.272 (29). 17994-17999 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Rezaul,K. et. al.: "Platelet-Activating Factor Stimulates Calcium-Dependent Activation of Protein-Tyrosine Kinase Syk in Human B Lymphocytes." Biochem. Biophys. Res. Commun.239. 23-27 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Soda,K. et. al.: "Relocation of Syk protein-tyrosine kinase to the actin filament network and subsequent association with Fak." Eur. J. Biochem.248. 827-833 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tubokawa,M. et. al.: "Interleukin-3 activates Syk in a human myeloblastic leukemia cell line, AML193." Eur. J. Biochem.249. 792-796 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoneya,H. et. al.: "Antibodies directed against ZAP-70 cross ract with a 66 kDa tyrosine kinase in the rat brain." Biochem. Biophys. Res. Commun.(in press).

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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