• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Novel clinical strategy for thoracic anerysm using intra/extra vascular stenting and molecular biological drug delivery system.

Research Project

Project/Area Number 09470277
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionOsaka University

Principal Investigator

HIRATA Nobuaki  Osaka University Medical School, Assistant Professor, 医学部, 助手 (70283752)

Co-Investigator(Kenkyū-buntansha) NISHIMURA Motonobu  Osaka University Medical School, Assistant Professor, 医学部, 助手 (90291442)
SAWA Yoshiki  Osaka University Medical School, Instructor, 医学部, 講師 (00243220)
OHTAKE Shigaeaki  Osaka University Medical School, Instructor, 医学部, 講師 (50243209)
MATSUDA Hikaru  Osaka University Medical School, Professor, 医学部, 教授 (00028614)
久米 よう一  大阪大学, 医学部・附属病院, 医員
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥11,900,000 (Direct Cost: ¥11,900,000)
Fiscal Year 1998: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1997: ¥8,200,000 (Direct Cost: ¥8,200,000)
Keywordsaortic aneurysm / vascular stent / drug delivery system / HVJ-liposome / gene tranfer / minimally invasive surgery
Research Abstract

Recently the number of patients with an aortic aneurysm has been growing with increasing population of old age. Operations such as graft replacement have so far been employed for treatment of an aortic aneurysm. but its clinical results are still not satisfactory in terms of both the operative mortality/morbidity and the postoperative quality of life. Thus it is important to develop a new strategy of minimally invasive approach for treatment of aortic aneurysm.
The purpose of this study is to prevent progression of aortic aneurysm by using endovascular drug delivery system (DDS) and to develop less invasive surgical procedure by introducing an endoscopic extravascular stenl in addition to an intravascular stent. As a molecular biological DDS, a gene transfection using HVJ-liposome method was used There is no report about extravascularstent for treatment of aortic aneurysm, and thus, this clinical strategy using intra/extra vascular stent combined with a new DDS using gene transfection i … More s considered to be a novel approach.
In the clinical study, the patients with a slight aneurysmal change in the aortic wall have been followad up in order to clarify and evaluate the factor of anerysmal formation. Encbvaseular stenting was performed in 4 patients with abdominal aortic aneurysm and 3 patients with thoracic aortic aneurysm with excellent results.
In the basic study, human senescent cell-derived inhibitor (sdi)-1 gene was transfected into the vein graft using HVJ-liposome method in rabbit vein graft model. Single intraluminal incubation of human sdi-1 gene resulted in significant inhibition of neointimal formation after vein grafting, associated whth phenotypic change of VSMCs from neonatal to adult type.
The correlation of the phenotypic modulation of smooth muscle cells in the process of neointima formation afer stent implation in rabbit aorta and leucine-rich small proteoglycans, decorin and biglycan, which are considered to participate in regulation of extracellular matrix accumulation in arterial intinal hyperplasia, is investigated. The biglycan and dccorin kinetics during neointima fomalion alter arterial injury are distinct ; biglycan synthesis correlates with the enbryonic smooth muscle cells, whereas decorin synthesis is accelerated by IL-1 beta secreted from macrophages accumulating in the injured areas in the late stage after the vascular injury.
These results may promise to clarify the mechanism of progression of aortic aneurysm and may help to develop a novel strategy of DDS in corporation with intra/extra stenting for treatment of aortic aneurysm. Less

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report

Research Products

(22 results)

All Other

All Publications (22 results)

  • [Publications] M.Nishimura: "Endovascular stent graft placement for patients with aortic aneurysm and end-organ dysfunction" ASAIO Journal. Vol 44 No.5. 511-515 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Sawa: "Efficient transfer of oligonucleotides and plasmid DNA into the whole heart through the coronary artery" Gene therapy. 5. 1472-1480 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hong-Zhi Bai: "Gene transfer to vein graft wall by HVJ-liposome method time course and localization of gene expression" Ann Thorac Surg. 66. 814-820 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hong-Zhi Bai: "Inhibition of intimal hyperplasia after vein grafting by in vivo transfer of human senescent cell-derived inhibition-1gene" Gene Therapy. 5. 761-769 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Imagawa: "Saarlar aneurysm in the nyht-sided aortic・arch A successfully corrected case" Journal of Vascular Surgery. 25. 941-944 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Taketani: "Idiopathic Iliae arterial aneurysm in a child" Journal of Pediatric Surgery. Vol 32 No.10. 1519-1521 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nishimura M,Ohtake S,Sawa Y,Imagawa H,Yamakawa T,Nakamura T,Maeda M,Tomoda K,Nakamura H,Matsuda H: "Endovasvular stent graft placement for patients with aortic aneurysm and end-organ dysfunction." ASAIO journal. 44. M511-M515 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sawa Y,Kaneda Y,Bai H,Suzuki K,Fujimoto J,Morishita R,Matsuda H: "Efficient transfer of oligonucleotides and plasmid DNA into the whole heart through the coronary artery." Gere therapy. 5. 1472-1480 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Bai H,Sawa Y,Zhang W,Yamakawa T,Morishita R,Kaneda Y,Matsuda H: "Gene transfer to vein graft wall by HVJ-liposome method : Time course and localization of gene expression." Ann Thorac Surg. 66. 814-820 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Bai H,Morishita R,Kida I,Yamakawa T,Zang W,Aoki H,Noda A,Nagai R,Kaneda Y,Higaki J,Ogihara T,Sawa Y,Matsuda H: "Inhibition of intimal hyperplasia after vein grafting by in vivo transfer of human senescent cell-derived inhibitor-1 gene." Gene therapy. 5. 761-769 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Imagawa H,Kadoba K,Taniguchi K,Sawa Y,Takahashi T,Fukushima N,Yoneda M,Fujii Y,Nakahara K,Matsuda H: "Saccular aneurysm in the right-sided aorticarch : A successfully corrected case." J Vasc Surg. 25. 941-944 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Taketani S,Imagawa H,Kadoba K,Sawa Y,Shirakura R,Matsuda H: "Idiopathic iliac arterial aneurysm in a child" J Pediatr Surg. 32. 1519-1521 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Bai H,Masuda J,Sawa Y,Nakano S,Shirakura R,SHimazaki Y,Ogata J,Matsuda H: "Neointima formation after vascular stent implantation : spatial and chronological distribution of smooth muscle cell proliferation and phenotypic modulation." Arterioscler Thromb. 14. 1846-1853 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamakawa T,Bai H,Masuda J,Sawa Y,Shirakura R,Ogata J,Matsuda H: "Differential expression of proteoglycans bigycan and decorin during neointima formation after stent implantation in normal and atherosclerotic rabbit aortas." (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Nishimura: "Endorascular steut graft placement for patients with aortic anenrysm and end-organ dysfunetion" ASAIO Journal. Vol44, No.5. 511-515 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y,Sawa: "Efficient transfer of oligonucleotides and plasmid DNA into the whole heart throngh the coronary artery" Gene therapy. 5. 1472-1480 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hong-zhi Bai: "Gene transfer to vein graft wall by HVJ-liposome method Time course and localization of gene expression" Ann thorac surg. 66. 814-820 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hong-zhi Bai: "Inhibition of intimal hyperplasia after nein grafting by in vivo transfer of human senescent cell-dertued inhibitor-1 gene" Gene therapy. 5. 761-769 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Iwazawa: "Succeelar aneurysm in the nyht-sided aortie arch A successfuley corrected cale" Journal of rascular surgery. 25. 941-944 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Taketani: "Idiopathic ltiae anterial aneurysm in a child" Journal of pediatric surgery. Vol32, No10. 1519-1521 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hiroshi Imagawa: "Saccular aneurysm in the right-sided aortic arch ; A successfully corrected case" Journal of vascular surgery. 25. 941-944 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Satoshi Taketani: "Idiopathic Iliac Arterial Aneurysm in a Child" Journal of Pediatric Surgery. Vol32 No10. 1519-1521 (1997)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi