The role of cytokines in varal infection and in allergy in the respiratory tract was evaluated.
Grant-in-Aid for Scientific Research (B).
|Research Institution||Yamanashi Medical University|
OKAMOTO Yoshitaka Professor in Department of Otolaryngology, Yamanashi Medical University, 医学部, 教授 (40169157)
菊島 一仁 山梨医科大学, 医学部, 助手 (70242659)
上條 篤 山梨医科大学, 医学部, 助手 (90252022)
MATSUZAKI Zensei Assistant professor in Department of Otolaryngology, Yamanashi Medical University, 医学部, 講師 (90283217)
MATSUOKA Tomokazu Directuer in Department of Otolaryngology, Yamanashi Medical University, 医学部, 助手 (30313810)
MIZUKOSHI Akihito Directuer in Department of Otolaryngology, Yamanashi Medical University, 医学部, 助手 (00283218)
|Project Fiscal Year
1997 – 1999
Completed(Fiscal Year 1999)
|Budget Amount *help
¥13,100,000 (Direct Cost : ¥13,100,000)
Fiscal Year 1999 : ¥2,800,000 (Direct Cost : ¥2,800,000)
Fiscal Year 1998 : ¥3,300,000 (Direct Cost : ¥3,300,000)
Fiscal Year 1997 : ¥7,000,000 (Direct Cost : ¥7,000,000)
|Keywords||Th1 cytokine / Th2 cytokine / respiratory syncytial virus / recombinant vacvina virus / nasal allergy / transgenic mice / RSウイルス / 鼻アレルギー / Th1サイトカイン / Th2サイトカイン / トランスジェニックマウス / 受動免疫 / 腸管免疫 / 鼻腔粘膜 / 抗体投与 / サイトカイン / 鼻腔免疫 / IL-10 / 鼻アレルギーモデルマウス / ワクチニアウイルス / 感染実験|
(1) The characteristics of the cytokines and immunity induced by viral antigen via different administration routes was comparatively evaluated. C57BL/6 mice were immunized via intranasal, intradermal, or enteric routes with a live recombinant vaccinia virus expressing the respiratory syncytial virus(RSV) G glycoprotein(r. Gvv) or F glycoprotein(r. FVV) and challenged intranasally with RSV Th2 cytokines were dominantly detected in the bronchoalveolar lavage of the mice immunized with r. GVV intradermally, and Th1 cytokines were dominant of the mice immunized with r. FVV intradermally, although their concentration was lower in the enteric and intranasal immunization groups. Resistance to RSV replication was observed in the lung of all groups of mice, however, in the nose only of intranasal immunization group. Lung inflammation characterized by infiltration of mononuclear cells and of eosinophils was strongest in the G.rVV intradermal immunization group, although observed in all groups to
(2) The role of IL-10 in nasal allergy and RSV infection was evaluated with transgenic mice which showed high expression of IL- 10 protein in nasal mucosa as well as in salivary glands.
After intranasal administration with RSV, viral replication in respiratory tract of the transgenic mice was significantly suppressed compared with that control non-transgenic mice. The suppression of viral replication was not observed in anti-IL-10 treated transgenic mice and Fas/Fas L system mediated CTL may be involved in the suppression of RSV replication observed in IL-10 transgenic mice.
In additional study, the transgenic mice were sensitized with ovalbumin. Nasal symptoms were stronger and Th2 cytokines production in nasopharyngeal lymphoid tissue and the number of eosinophils in nasal mucosa were found to be significantly higher in transgenic mice compared with those in control mice. IL-10 may affect on the development of the dysregulation of Th1/Th2 cytokines.
IL-10を鼻粘膜組織で強く発現させたトランスジェニックマウス(以下IL-10 TG)を用いて鼻アレルギー、上気道ウイルス感染へのIL-10の役割を検討したところ、IL-10を鼻粘膜局所で強く発現させたマウスでは、感染細胞でのアポトーシスの誘導亢進、アレルギー感作でのTh2/Th1サイトカインのアンバランス亢進など多彩な変化が認められた。 Less
Research Output (16results)