| Budget Amount *help |
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1998: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1997: ¥5,700,000 (Direct Cost: ¥5,700,000)
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| Research Abstract |
Though active oxygen scavenging activity is known to prevent life-style related diseases, it is not clear how food components with this activity change during cooking and how active they are in the served meals. However, there is no convenient method to determine the active oxygen scavenging activity in food. In this work, the investigators used the scavenging activity against 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical as the index of active oxygen scavenging activity. When DPPH is trapped by substances with active oxygen scavenging activity, it changes to non-radical form and lose visible absorption. However, spectrophotometer cannot detect this change in foods because foods are rich in colored substances such as pigments. Therefore, the investigators developed DPPH-HPLC method, which determines DPPH separately, and enabled the determination of active oxygen scavenging activity in foods. Then, the active oxygen scavenging activity of cabbage and Chinese cabbage grown in different conditions was determined. There was no significant difference between the activities of vegetables grown with chemical fertilizer and organic fertilizer. Finally, the changes of active oxygen scavenging activity during cooking of curry were determined. The activity of vegetables increased by heat, while the activity of spices decreased. In cooked curry, the level of the activity from vegetables was similar as that from spices. In conclusion, the method for the determination of active oxygen scavenging activity in foods was successfully developed, and the changes in active oxygen scavenging activity during cooking were clarified. These results contribute the planning of dietary life to keep health.
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