| Project/Area Number |
09480217
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
KOIKE Tatsuro Hokkaido Univ., Grad. School of Sci., Professor, 大学院・理学研究科, 教授 (80128131)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Shuuitsu Hokkaido Univ., Grad. School of Sci., Instructor, 大学院・理学研究科, 助手 (90202431)
WATANABE Masahiko Hokkaido Univ., Grad. School of Med., Professor, 大学院・医学研究科, 教授 (70210945)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥9,300,000 (Direct Cost: ¥9,300,000)
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| Keywords | Microglia / Apoptosis / Cerebellum / Granule neuron / lysosome / ネクローシス / 小脳発達 / グルタミン酸 / ノーザンブロット / 免疫組織化学 / 活性化 / 哺食 / 小脳顆粒細胞 / アクソトミ-(神経繊維切断) |
|---|
| Research Abstract |
To searach for genes up-regulated during neuronal cell death, we have employed cerebellar cell cultures, in which glial cells respond to degeneration and cell death of granule neurons that begins to occur at 4DIV. Differential hybridization was performed with subtracted cDNA probes and a cDNA library from 5DIV. One of the genes up-regulated during cell death is a novel gene, mrf-1, in microglia. Another gene is highly homologous to the mouse genes with hematopoietic cell origin which may be involved in microglial activation associated with enhanced functions of lysosomes
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