| Project/Area Number |
09556066
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B).
|
| Allocation Type | Single-year Grants |
|---|
| Section | 展開研究 |
|---|
| Research Field |
Applied veterinary science
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
KAI Chieko LABORATORY ANIMAL RESEARCH CENTER, INSTITUTE OF MEDICAL SCIENCE, THE UNIVERSITY of TOKYO, 医科学研究所, 教授 (10167330)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
AIDA Yoko RIKEN TSUKUBA INSTITUTE, ライフサイエンス筑波研究センター, 研究員 (50182994)
KOFUNE Tomio DEPARTMENT OF PATHOLOGY, NATIONAL INSTITUTE OF HEALTH AND WELFARE, 病理部, 主任研究官 (80142644)
SAITO Izumu LABORATORY OF MOLECULAR GENETICS, INSTITUTE OF MEDICAL SCIENCE, THE UNIVERSITY OF TOKYO, 医科学研究所, 教授 (70158913)
YAMANOUCHI Kazuya NIPPON INSTITUTE FOR BIOLOGICAL SCIENCE, 研究部, 主任研究員 (30072888)
宮沢 孝幸 東京大学, 大学院・農学生命科学研究科, 助手 (80282705)
|
|---|
| Project Period (FY) |
1997 – 2000
|
| Project Status |
Completed (Fiscal Year 2000)
|
| Budget Amount *help |
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 2000: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1997: ¥3,600,000 (Direct Cost: ¥3,600,000)
|
|---|
| Keywords | adenovirus / canine distemper / CDV / H protein / CTL / アデノイルス / 牛疫 / 細胞性免疫 / ウシ白血病ウイルス / MHC / 牛疫ウイルス / ウサギ / 細胞傷害試験 / 組み換えアデノウイルス / lacZ / 牛白血病ウイルス / ウシ |
|---|
| Research Abstract |
The AIM of this research is the development of analysis method of immune surveillance system in various animals by using adenovirus vector to characterize cell mediated immunity surveillance system. The various results were obtained, as followings,
(1) We established the recombinant adenovirus vector encoding canine distemper virus (CDV) hemagglutinin (H) gene. We also established the method to get primary dog skin cells for the target of cytotoxic T lymphocyte (CTL) assay. It makes us possible to get the targeted cells from self-animals.
(2) We identified epitopes for cell-mediated immunity and humoral immunity in N protein of rinderpest virus. By utilizing immune-stimulating complex (ISCOM), recombinant H protein was revealed to be effective in cell mediated immunity.
(3) We identified the resistant Major Histocompatibility Complex (BoLA) class II molecules to the bovine leukemia ; BoLA-DR3 allele. The recombinant adenovirus encoding BLV genome
|
