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Introduction of exogenous proteins into nucleus by using anthrax toxin

Research Project

Project/Area Number 09557025
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Bacteriology (including Mycology)
Research InstitutionInstitute of Life Science, Kurume University

Principal Investigator

MEKADA Eisuke  INSTITUTION,DEPARTMENT,TITLE OF POSITION PROFESSOR, 分子生命科学研究所, 教授 (20135742)

Co-Investigator(Kenkyū-buntansha) UMATA Toshiyuki  INSTITUTION,DEPARTMENT,TITLE OF POSITION ASSISTANT PROFESSOR, 分子生命科学研究所, 助手 (30213482)
TSUNEOKA Makoto  INSTITUTION,DEPARTMENT,TITLE OF POSITION ASSISTANT PROFESSOR, 分子生命科学研究所, 講師 (50197745)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥10,700,000 (Direct Cost: ¥10,700,000)
Fiscal Year 1998: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1997: ¥7,700,000 (Direct Cost: ¥7,700,000)
Keywordsanthrax toxin / GFP / recombinant proteins / 色素性乾皮症 / 蛋白質導入
Research Abstract

Anthrax toxin is comprised of functionally different two components. Lethal factor (LF) is a effector molecule, while protective antigen (PA) is a molecule which facilitates the ently of LF into cytosol. The N-terminal part of LF molecule is binding domain to PA.Fusion proteins containing the N-terminal part of LF has been shown to be translocated to the cytosol by means of the activity of PA.In this study we attempted to make a recombinant proteins which were translocated into nuclei from outside of cells by interaction with PA.We have constructed the fusion proteins comprising GFP and N-terminal hexsa lysine complex. However, this protein was quite unstable and it did not permit to analyze the translocation of the fusion proteins. Constructions of other form of fusion proteins are in progress.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Izumi, Y.: "A metalloprotease-disintegrin, MDC9/Meltrin-g/DM9, and PKCd are involved in TPA-induced ecftodomain sheddding of membrane-anchored heparin-binding EGF-like growth factor." EMBO J.17. 7260-7272 (19978)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsuneoka, M.: "N-myc transactivates RCCI gene expression in rat firoblast cells transformed by N-myc and v-ras." J.Biochem.124. 1013-1019 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Umata, T.: "Diphtheria toxin translocation across endosome membrane. A novel cell permeabilization assay reveals new diphtheria toxin fragments in endocytic vesicles." J.Biol.Chem.273. 8351-8359 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mitamura. T.: "Structure-function analysis of the diphtheria toxin receptor toxin binding site by site-directed mutagednesis." J.Biol.Chem.272. 27084-27090 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsuneoka, M.: "c-myc activates RCCI gene expression through E-box eledmdents." Oncogened. 14. 2301-2311 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kagawa, T.: "Immune system-related CD9 is expressed in mouse cdentral nervous system myelin at a very late stage of myelination." J.Neurosci. Res.50. 312-320 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 岩本 亮: "医学&サイエンスシリーズ 細胞接着のしくみと疾患" 羊土社, 7 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Izumi, Y.: "Ametalloprotease-disintegrin, MDC9/Meltrin-g/ADM9, and PKCd are involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor." EMBO J.17. 7260-7272 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsuneoka, M.: "N-mycc transactivates RCC1 geneexpression in rat fibroblast cells transformed by N-myc and v-ras." J.Biochem.124. 1013-1019 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Umata, T.: "Diphtheria toxin translocation across endosome membrane. A novel cell permeabilization assay reveals new diphtheria toxin fragments in endocytic vesicles." J.Biol.Chem.273. 8351-8359 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mitamura, T.: "Structure-function analysis of the diphtheria toxin receptor toxin binding site by site-directed mutagenesis." J.Biol.Chem.272. 27084-27090 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsuneoka, M.: "c-myc activates RCC1 geneexpression through E-box elements" Oncogene. 14. 2301-2311 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kagawa, T.: "Immune system-related CD9 is expressed in mouse central nervous system myelin at a very late stage of myelination." J.Neurosci. Res.50. 312-320 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Izumi, Y.: "A metalloprotease-disintegrin, MDC9/Meltrin-g/ADM9, and PKCd are involved in TPA- induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor." EMBO J.17. 7260-7272 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsuneoka, M.: "N-myc transactivates RCCl gene expression in rat fibroblast cells transformed by N-myc and v-ras." J. Biochem.124. 1013-1019 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Umata, T.: "Diphtheria toxin translocation across endosome membrane. A novel cell permeabilization assay reveals new diphtheria toxin fragments in endocytic vesicles." J. Biol. Chem.273. 8351-8359 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 岩本 亮: "医学&サイエンスシリーズ 細胞接着のしくみと疾患" 羊土社, 7 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mitamura,T.: "Structure-Function Analysis of the Diphtheria Toxin Receptor Toxin Binding Site by Site-directed Mutagenesis." J.Biol.Chem.272. 27084-27090 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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