|Budget Amount *help
¥2,500,000 (Direct Cost : ¥2,500,000)
Fiscal Year 1999 : ¥1,300,000 (Direct Cost : ¥1,300,000)
Fiscal Year 1998 : ¥1,200,000 (Direct Cost : ¥1,200,000)
By using gas chromatography-mass spectrometry, trace volatile hydrocarbon analysis in blood of fire victim is very useful for elucidating the conditions prevailing before death. Also the analysis provides useful information for discriminating between gasoline and kerosene which are often used in ignition. However, the methods so far provided had not been available for practical use. The GC-MS instruments should be used under specialized conditions for convenient analysis of volatile hydrocarbons. In practical, we have many other works using the GC-MS. So that, we need to provide a practical method in which the instruments can be commonly used for various analyses. In this study, we attempted to characterize the analytical profiles of blood analysis in practical cases and to develop a practical method.
1. Indicators to characterize components of combustion gas and petrolic gases detected in blood analysis
Combustion gas (from common stuffs) : Stylene, benzene, toluene.
Gasoline : pentane, hexane, heptane, and aromatic hydrocarbons ranging from C6 to C9.
Kerosene : octane, nonane, decane, dodecane, tridecane, and aromatic hydrocarbons ranging C6 to C9.
2. Development of practical method
Solid-phase micro-extraction of volatile components in low-temperature headspace was newly introduced for retaining light volatile compounds in an SPME fiber. The gas chromatographic conditions were set for concentrating those at a starting part of capillary column under - 40℃. Under the acquired conditions, the indicators excepting pentane were detected in the practical levels.
Accordingly, hydrocarbon analysis indicated in this study is practically useful for investigating fired case.