|Budget Amount *help
¥3,800,000 (Direct Cost : ¥3,800,000)
Fiscal Year 1998 : ¥1,300,000 (Direct Cost : ¥1,300,000)
Fiscal Year 1997 : ¥2,500,000 (Direct Cost : ¥2,500,000)
gamma-Hexachlorocyclohexane (gamma-HCH, BHC, and lindane) is one of several highly chlorinated insecticides that cause serious environmental problems. The cellular proteins of a gamma-HCH-degrading bacterium Sphingomonas paucimobilis UT26 were fractionated into periplasmic, cytosolic and membrane fractions after osmotic shock. Most of activities of LinA, LinB, LinC, LinD, and LinE that are involved in the early steps of gamma-HCH degradation in UT26, were detected in the periplasmic fraction. Antibodies were raised against LinA and LinB.Most of the LinA and LinB protein was detected in the periplasmic fraction and had not undertaken molecular processing. Furthermore, immunoelectron microscopy clearly showed that LinA and LinB are periplasmic proteins. LinA and LinB both lack a typical signal sequence for export, so they may be secreted into the periplasmic space via a hitherto unknown mechanism.