|Budget Amount *help
¥2,800,000 (Direct Cost : ¥2,800,000)
Fiscal Year 1998 : ¥1,100,000 (Direct Cost : ¥1,100,000)
Fiscal Year 1997 : ¥1,700,000 (Direct Cost : ¥1,700,000)
(1) Glucosone (GLCO), 3-deoxyglucosone (3DG), glyoxal (GO), and methyiglyoxal (MG) were detected in the reaction systems of lysozyme-glucose (LG) and lysozyme-fructose (LF). Transition metals gave significant effects on the formation of GLCO and GO.On the other hand, oxygen was not essential for the generation of 3DG.(2) Novel imidazole compound was identified by the reaction of 3DG and N -benzoylarginine amide. Lysyl-pyrropyridine was identified in the reacton of lysozyme and reducing sugars.
(1) The amounts of lysyl-pyrropyridine and pentosidine were determined in LG, LE, and L-3DG reaction systems. Lysyl-pyrropyridine increased in quantity compared to pentosidine in 3DG-lysozyme reacton systems. In LG and LF reaction systems, pentosidine increased in comparison with the formation amounts of lysyl-pyrropyridine.
(2) 3DG reacted readily with 2'-deoxyguanosine in nucleosides. Two major products (G-A and G-B) were isolated and purified from the reaction mixture of 3DG and 2'-deoxyguariosine. G-A was identified as N- (1-oxo-2,4,5,6-hydroxyhexyl) -2'- deoxyguanosine. G-B was identified as a diastereomer of G-A.
(2) 3DGを生理的条件下、50℃で反応では各種ヌクレオシドと反応させた結果、2'-deoxyguanosine(dG)との反応性が最も高かった。その主要な反応生成物(GA,GB)を3DGとdGがアミド結合した、N-(l-oxo2,4,5,6-hydroxy)-2'-deoxyguanosineのジアステレオマーと同定した。また、3DGとcalfthymusDNAの反応によってもGA,GBが検出された。また、3DGと同様な2-オキソアルデヒド(2-OA)であるグリオキサール(GO)の10μM添加以上で肝臓細胞に対し、細胞毒性が認められ、発ガンマーカーであるgulutathione-S-transferase mRNAも増大した。