| Project/Area Number |
09670016
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | The University of Tokushima |
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Principal Investigator |
ISHIMURA Kazunori The University of Tokushima, School of Medicine, Department of Anatomy, Professor, 医学部, 教授 (90112185)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | l7alpha-Hydroxylase / C17-20 1yase / Steroid hormones / Transcription factor / Immunohistochemistry / Electron microscope / Testis / Liver / Placenta / (in press) / アポトーシス / プロスタグランジン / PbX1 |
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| Research Abstract |
(1) Expression of PbX1, a transcription factor of 17 alpha-hydroxylase/C17-20 lyase, was studied in the rat gonads, adrenal cortex and placenta. Only in the placenta, both the transcription factor and the enzyme were co-expressed.
(2) 17alpha-Hydroxylase/G17-20 lyase was transiently expressed in the hepatocytes of 1 to 3 week-old rats. The expression of the enzyme in the liver may be involved in the onset of puberty.
(3) Keratinocytes overexpressing 12-lipoxygenase enzyme showed structural changes of the cell nuclei. The enzyme may relate to keratinization.
Apoptotic cells in the rat testis were examined by immunohistochemistry with an antibody against single-stranded DNA and in situ terminal deoxynucleotidyl transferase staining.
The results suggested that fetal Leydig cells undergo apoptosis during the perinatal period. NADPH-diaphorase and vasoactive intestinal peptide were colocalized in the penile cavernous tissues.
(4) Prostaglandin F synthase was immunohistochemically localized in the dendrites of nerve cells in the rat central nervous system.
Prostaglandin F synthase was immunohistochemically localized in the vascular endothelial cells of the rat various tissues.
12-Lipoxygenase was immunohistochemically localized in the serotonin-containing entero-endocrine cells of the mouse gastrointestinal tract. The enzyme may be involved in the hormone release.
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