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CHARACTERIZATION OF RAT THERMOSTATS

Research Project

Project/Area Number 09670069
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KOBAYASHI Shigeo  Graduate of School of Informatics, Kyoto University, Professor, 情報学研究科, 教授 (40124797)

Co-Investigator(Kenkyū-buntansha) SATO Masahiko  Faculty of Integrated Human Studies, Kyoto University, Assistant, 総合人間学部, 助手 (20283575)
TAEYASU Kunihiko  Faculty of Integrated Human Studies, Kyoto University, Professor, 総合人間学部, 教授 (40135695)
ISHIHARA Akihiko  Faculty of Integrated Human Studies, Kyoto University, Associate Professor, 総合人間学部, 助教授 (90184548)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsTHERMOSTAT / CLONING / WARM / CHANNEL / mRNA / 発現クローニング / cDNAライブラリー / 後根神経節 / 視床下部 / 卵母細胞 / 冷受容ニューロン / 温度受容ニューロン / F-11
Research Abstract

We have identified that warm sensitive cells in the hypothalamus and warm-receptors in the skin act as thermostats for thermoregulation. Patch clamp studies of hypothalamic warm-sensitive neurons suggest that warming-activated cation channel is key factor of the thermostats. The purpose of this study is molecular cloning of genes relevant to the thermostat. We used F-11 (cell line derived from dorsal root ganglion) as a source of mRNA.
1. Warming increased intracellular CaィイD12+ィエD1 level [CaィイD12+ィエD1]I in F-11 cells, detected by Ca Fura-2AM.
2. Warming induced whole-cell inward current in F-11 cells voltage clamped at -60mV.
3. Oocytes infected with F-11 mRNA responded to warming, detected by two-electrode voltage-clamp method. First, warming induced current continuously Second, warming induced current transiently and inactivated soon.
We plan to make f-11 cDNA library for molecular cloning of genes relevant to thermostats.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] Shigeo Kobayashi: "Warming activated channels of warm-sensitive neurons in rat hypothalamic slices"Neuroscience Letters. 273. 1-4 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 小林茂夫: "生体情報処理"コロナ社. 200 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shigeo Kobayashi: "Warming activated channels of warm-sensitive neurons in rat hypothalamic slices"Neuroscience Letters. 273. 1-4 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] KOBAYASHI, Shigeo: "Seitai Joho Shori"Korona-sha. 200 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 小林茂夫: "生体情報処理-生体サーモスタットから見えてくる生体情報系" コロナ社, 230 (2000)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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