|Budget Amount *help
¥3,600,000 (Direct Cost : ¥3,600,000)
Fiscal Year 1998 : ¥1,600,000 (Direct Cost : ¥1,600,000)
Fiscal Year 1997 : ¥2,000,000 (Direct Cost : ¥2,000,000)
NKR-P1 molecule is thought to be one of activating receptor for NK cells and suggested to play an important role in cytotoxicity and IFN-gamma production by NK cells. Therefore, it is important to clarify the signaling mechanism of NKR-P1 molecule. In this study, in order to clarify the signaling pathway for NKR-P1 molecule, we analyzed association molecule for NKR-P1 and found that FcRgamma chain is associated with NKR-P1. Furthermore, analysis using FcRgamma-deficient mice revealed that FcRgamma is essential for signal transduction of NKR-P1.
On the other hand, NK cells are known to express CD3zetachain, a signal transducing molecule for TCR.However, the role of GD3zetaon NK cells has remained unclear, In order to analyze the function of CD3zeta expressed on NK cells, we purified NK cells from CD3zeta deficient mice and analyzed expression of various surface molecules. Then, we found that expression of FcgammaRIII is significantly high on NK cells from CD3zeta-deficient mice compared with that from normal mice. Furthermore, NK cells from CD3zeta-deficient mice produced a large amount of IFN-gammacompared with those from normal mice upon stimulation with FcgammaRIII crosslinking. These findings showed that CD3zetachain has an important role for the regulation of expression of FcgammaRIII on NK cells.