| Project/Area Number |
09670416
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Public health/Health science
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| Research Institution | Nippon Medical School |
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Principal Investigator |
SUZUKI Hiroshi Nippon Medical School, Institute of gerontology, Assistant Professor, 老人病研究所, 講師 (70142528)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIO Osamu Institute of Public Health, Dept. of Microbiology, Chief of virology, 衛生微生物, ウイルス室長 (40270631)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Norwalk-like virus / Human Calicivirus / varial comtamination / Oyster / Scallop / food poisoning incident / gastroenterits |
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| Research Abstract |
Recently, the nonbacterial gastroenteritis caused by contaminated shellfish occurs frequently. To have a clear grasp the condition of contamination, we had collected oysters in N prefecture and scallops in A prefecture at each month for a long period including in summer when they are not eaten in raw condition. Moreover, we had also searched oysters on the market in winter. The objects of virus were Norwalk-like-virus (NLVs), Astrovirus and Hepatitis A virus.
From September 1995 to 1999, NLVs RNA was detected from 16 in 46 specimens (34.8%) of oysters from N prefecture by RT-PCR. Six were genotypte 1, 5 were genotype 2 and 5 were not untypable.
On the scallops from A prefecture, NLVs RNA was detected for the first time in February and March 2000 since we started to search in October 1995. They were genotype 2. Astrovirus and Hepatitis A virus were not detected.
From 46 oysters on the market, 9 of NLVs RNA were detected (19.6%). Seven were genotype 2 and 2 was not untypable. Astrovirus was detected from 1 specimen. Hepatitis A virus was not detected.
There were food poisoning incidents for ski tourists from 3 different prefectures, who stayed at hotel in N prefecture, in January 2000. Public health center in each prefecture detected NLVs RNA from their fecal specimens. We analyzed nucleotide sequence from those, and that was genotype 2 in spite of having a little differences. Viral contaminated oyster had a great possibility as a cause of ood poisoning incidents.
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