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Role of interferon-gamma in development of myositis : generation and analysis of murine myositis by DNA vaccination

Research Project

Project/Area Number 09670467
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionChiba University

Principal Investigator

KURASAWA Kazuhiro  Chiba University, School of Medicine, Assistant, 医学部, 助手 (30282479)

Co-Investigator(Kenkyū-buntansha) IWAMOTO Itsuo  Chiba University, School of Medicine, Associate Professor, 医学部, 助教授 (10111436)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsPolymyositis / DNA vaccination / immunologicam mechanism / cytokines / interferon-gamma / ICAM-1 / Interferen-γ / 副刺激 / 接着分子
Research Abstract

To investigate the role of interferon-gamma (IFN-gamma) in development of Polymyositis, we expressed IFN-gamma and beta-galactosidase (beta-Gal) in muscle of mice by injection of naked DNA encoding these proteins, DNA vaccination. beta-Gal was expressed in 2-5% of the muscle in mice injected with the plasmid. These mice expressing beta-Gal showed cellular and humoral immune response to beta-Ga1, indicated by proliferative response of splenocytes and serum antibodies to beta-Gal. However, no or very mild cell infiltration was observed in the muscle expressing beta-Gal. In addition, myositis was not found in mice injected with plasmid encoding IFN-gamma. In contrast to these mice, mice inoculated with both beta-Gal and IFN-gamma developed inflammation of the injected muscle. Furthermore, mice that injected with beta-Gal- and IFN-gamma- expression vector into right lower limb and with only beta-Gal- plasmid into left limb developed myositis of the muscle inoculated with both plasmids. In addition, treatment with anti-ICAM-1 mAb partially suppressed myositis induced by DNA vaccination. These findings indicates that IFN-gamma at the site of inflammation plays an important role in development of myositis.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Sano H et al: "Cross-linking of ICAM-linduces IL・8 and Ranles Production Fhorogh the activatim of MADKince in huminsolm endothid cars" Biochem Biophys Res Cominun. 250. 694-698 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kasai M et al: "Blakade of CD28/87 internctein Suppresses alkryic eosrnophlic in flammation in mice" Int Arch Allergy Immunol. 117 Suppl1. 14-19 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakao et al: "High-dase oral fokrance prevents antigen-induced eosinophl recratment ulo mouse aran〓" Int.Immunol.10. 387-394 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kurosawa K et al: "Fan modulates both positure and negalne selection of thymocytes" Cell Immunol. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nawata Y et al: "Corticosterdid resistant Interstilial Preunomhsin Dermafomyositis/Polymyositis Reclictrin and Trentant anithcyelospase" J.Rheumafl. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sano H et al: "Cross-linting of ICAM-1 induces IL-8 and Rantes poroduction through the activetion of MAP kinax in hama sesutis exdottelind celts" Biochen Biophys Res Commun. 250. 694-/698 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kasai M et al: "Blakade of CO28/87 interction suppresses allergic eosinephilic in flammation in mue" Int Arch Allergy Immunol.117 Suppl1. 14-19 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Vakao et al: "High-dose oral tokrance prevents antigen-induced eosinophl recruitment into movse sirays" Int.Immunol.10. 387-394 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kurosawa K et al: "Fas modulates both positue and nenegalne seleclion of thymeytes" Cell Immunol. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Naweta Y et al: "Corticosleroid resislant Inter stihal Penumonihsin Derma fomyositisl Polymyisitia:Preclidion and Treatment Cuithcyclospors" J Rheumofol. (in press). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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