| Project/Area Number |
09670616
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | THE UNIVERSITY OF TOKUSHIMA |
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Principal Investigator |
YASUOKA Susumu SCHOOL OF MEDICAL SCIENCES,THE UNIVERSITY OF TOKUSHIMA,PROFESSOR, 医療技術短期大学部, 教授 (30035414)
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| Co-Investigator(Kenkyū-buntansha) |
SANO Toshiaki THE UNIVERSITY OF TOKUSHIMA,SCHOOL OF MEDICINE PROFESSOR, 医学部, 教授 (80154128)
KOYAMA Hajime THE UNIVERSITY OF TOKUSHIMA,SCHOOL OF MEDICINE ASSITENT PROFESSOR, 医学部, 助教授 (80109074)
NAKAMURA Yoichi THE UNIVERSITY OF TOKUSHIMA,HOSPITAL OF SCHOOL OF MEDICINE, LECTURER, 医学部附属病院, 講師 (00237447)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | HUMAN AIRWAY TRYPSIN-LIKE PROTEASE / AIRWAY / ACTIVATION OF PROUROKINASE / HYDROLYSIS OF FIBRINOGEN / AIYWAY EPITHELIAL CELLS / IMMUNOHISTOCHEMISTRY / INFLUENZA VIRUS / HEMAGGLUTININ / フィブリノーゲンの分解 / トリプシン様プロテアーゼ(トリプターゼ) / 遺伝子 / プラスミノーゲンアクチベータ-様プロテアーゼ / トリプシン |
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| Research Abstract |
IN ORDER TO CLARIFY PATHOPHYSIOLOGICAL SIGNIFICANCE OF HUMAN AIRWAY TRYPSIN-LIKE PROTEASE(HAT), WHICH I WE FOUND IN THE HUMAN AIR WAY, IN AIRWAY INFLAMATION, FOLLOWING RESERCHES WERE CARRIED OUT.
1. LOCALIZATION OF FIAT IN AIRWAYS : LOCALIZATION OF HAT IN THE AIRWAYS WAS IMMUNOHISTOCHEMICALLY EXAMINED USING FRESH BRONCHIAL TISSUES OBTAINED AT SURGERY IT WAS SHOWN THAT POSITIVE REACTION WAS FOUND IN BRONCHIAL EPITHELIAL TISSUES.THIS RESULT INDICATES THAT HAT IS BIOSYNTHESIZED IN AIRWAY EPITHELIAL CELLS.
2. ABILITY TO HYDROLYZE ENDOGENOUS PROTEINS OF FIAT : IT WAS SHOWN BY SDS-POLYACRY LAMIDE GEL ELECTROPHORESIS THAT HAT CONVERTS PROUROKINASE INTO UROKINASE BY LIMITED PROTEOLYSIS, AND THAT HAT CLEAVES FIBRINOGEN, ESPECIALLY ITS Ephi-CAIN.
3. EFFECT OF I HAT ON INFLUENZA VIRUS IN VITRO : HAT DID NOT CLEAVE HEMOAGGLUTININ OF INFLUENZA VIRUS, WHILE BOVINE TRYPSIN CLEAVED.
4. EFFECT OF HAT ON AIRWAY EPITHELIAL CELLS : IT WAS SHOWN IN VITRO SYSTEM THAT HAT PROMOTES GROWTH OF BEAS-2 CELLS, AND IT ALSO PROMOTES PRODUCTION OF INTERLEUKIN-8 AND GM-CSF BY BEAS-2 CELLS.
5. CONCLUSION : IT IS POSTULATED THAT HAT IS BIOSYNTHESIZED IN AIRWAY EPITHELIAL CELLS, AND IS RELATED TO BIOLOGICAL DEFENCE MECHANISM OF AIRWAY MUCOSAL MEMBRANE.FOR INSTANCE, HATIS THOUGHT TO BE RELATED TO BREACKDOWN AND REGENERATION OF AIRWAY MUCOUS MEMBRANE VIA PROCESS OF ACTIVATION OF PROUROKINASE.ACTIVATION OF PLAMINOGEN, ACTIVATION OF METAL PROTEASE SUCH AS COLLAGENASE.HOWEVER, IT IS ALSO POSTULATED THAT FIAT IS RELATED TO AMPLIFICATION OF REMODELING OF AIRWAY TISSUES IN CRONIC INFLAMATORY DISEASES WHERE OVERPRODUCTION OF HAT OCCURS.
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