|Budget Amount *help
¥3,000,000 (Direct Cost : ¥3,000,000)
Fiscal Year 1998 : ¥800,000 (Direct Cost : ¥800,000)
Fiscal Year 1997 : ¥2,200,000 (Direct Cost : ¥2,200,000)
1. Polymerase chain reaction (PCR) method was developed to identify septicemia caused by staphylococcus, group B streptococcus, yersinia, mycoplasma and mycobacterium.
2. Serological method for identification of Bartonella henselae infection was developed.
1) A total of4l patients out of 119 patients with having suspicion of cat scratch disease were serologically diagnosed as cat scratch disease. Of these 41 patients, 14 had systemic Bartonella henselae infection including fever of unknown origin, granuloma of the liver and/or spleen.
2) The prevalence of antibodies against Bartonella henselae among Japanese was 6.4% (17/267).
3) The prevalence of antibodies against Barlonella henselae was 30% (6/20) in domestio cats, and 7.6% (4/52) in domestic dogs.
4) Bartonella henselae infections caused by domestic dogs were identified in two patients, suggesting that domestic dog as well as cat can serve as a potential reservoir of Bartonella henselae in man, and the owner of such dog might be at risk to have Bartonella henselae infections.
3. PCR method to detect Barionella henselae Bartonell was developed.
1) The prevalence of Bartonella henselae DNA in the peripheral blood in cats and dogs were 6.2% (1/16), and 5.7% (3/52), respectively.
2) The prevalence of Bartonella henselae DNA in the oral swabs in cats and dogs were 12.5% (2/16), and 55.5% (5/9), respectively.