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EFFECT OF GROWTHFACTOR (S) ON CEREBRAL HISTOGENESIS : ANALYSIS ON TISSUE CULTRED EXPLANT

Research Project

Project/Area Number 09670834
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionKEIO UNIVERSITY

Principal Investigator

TAKAHASHI Takao  KEIO UNIVERSITY,SCOOL OF MEDICINE Dept.of PEDIATRICS ASSISITANT PROFESSOR, 医学部, 講師 (80171495)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsCerebral Histogenesis / Mouse / Cell Cycle / Tissue Culture / Gap Junction / Basic Fibroblast Growth Factor / Octanol / 分化 / 大脳皮質発生 / 細胞周期 / 組織培養
Research Abstract

Analysis of cell cycle regulation in neocortical neuronogenesis has been undertaken in vitro in the embryonic day 13 mouse. Explants of the dorsomedial region of the cerebral wall, implanted in collagen gel, were incubated for 1 hr in minimal medium and then in minimal medium containing BUdR.After 30 mm with BUdR (1.5 hr in vitro), the labeling index (LI, labeled/total cells) was 0.41 as compared to the previously determined LI of 0.38 in vivo. The in vitro LI remained at 0.4 over the next 7 hr of continued exposure to BUdR and then ascended linearly to asymptote at 0.73. These observations establish (1) that there is a flux of cells from G1 to S for the first 1.5 hr in vitro, (2) that this G1 to S flux is then interrupted for the following 7 hr but (3) that G1 to S flux is subsequently reestablished. In this same system, mitotic figures were first labeled only after a 5 hr exposure to BUdR but then over 2 more hours all mitoses became labeled. In vivo, by contrast, all mitoses become … More labeled during 2 hr of BUdR exposure. By implication there is a transient arrest in the progression from S to M phase which is evidently overcome within 5 hr of explantation. The duration of the reestablished cell cycle and those of G1, Sand combined G2-M phases were estimated to be 19.2, 6.3 -8.3, 8.8, and 2.0 -4.0 hr, respectively. The leaving (Q) fraction of the cycle (0,64) was twice the in vivo value. Two thirds of the Q fraction cells remained in the ventricular epithelium, resulting in a substantially low growth fraction of 0.73 as compared with 1.0 in vivo.
Also examined in a cerebral wall explant in vitro was the consequences of basic fibroblast growth factor (b-FGF) and the gap junction uncoupling agent, 1-octanol. LI was determined in the sector of the medial and lateral cortical zones, corresponding to areas 1 and 40, respectively. In area 40, the overall LI in b-FGF-exposed explants was higher and that in 1-octanol-exposed explants was lower than the LI in control explants. Those observations suggest that b-FGF reduces Q fraction while 1-octanol increases the fraction. Less

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Takahashi.T, Bhide, PG, Goto, T, Miyama, S and Caviness, VS: "Proliferative behavior of the murine cerebral wall in tissue culture : cell cycle kinetics and checkpoints." Experimental Neurology. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takahashi.T, Nowakowski, RS and Caviness, VS: "Cell cycle as operational unit ne of ocortical neuronogenesis." Neuroscientist. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Caviness, VS, Jr., Takahashi.T and Nowakowski, RS: "The G1 restriction point as critical regulator of neocortical neuronogenesis." J Neurochem Res. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Caviness, VS.Jr., and Takahashi.T: "La cytogenese neocorticale : le gradent proliferatif de l'epithelium pseudostratifie ventriculaire (PVE) et histogenese du cortex cerebral." Percentiel. vol 3. 5-10 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takahashi.T, Nowakowski, RS and Caviness, VS, Jr.: "Mathmatics of neocortical neuronogenesis." Developmental Neuroscience. vol 19. 17-22 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miyama S, Takahashi.T, Nowakowski RS, Caviness, VS, Jr.: "A gradient in the duration of the G1 phase in the murine neocortical proliferative epithelium." Cerebral Cortex. vol 17. 678-689 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Caviness, VS, Jr., Takahashi.T and Nowakowski, RS: "Cell Proliferation in cortical development. In : Normal and abnormal development of the cortex." Eds : Galaburda AM and Christen Y. Springer-Verlag, 199 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takahashi.T, Miyama, S and Caviness, VS, Jr.,: "Regulated parameters of cell proliferation in neocortical histogenesis. In : Neural Development" Eds : K Uyemura, K.Kawamura and T.Yazaki Springer-Verlag, 544 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takahashi, T,Bhide, PG,Goto, T,Miyama, S and Caviness, VS: "Proliferative behavior of the murine cerebral wall in tissue culture cell cycle kinetics and checkpoints" Experimental Neurology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takahashi, T,Nowakowski, RS and Caviness, VS: "Cell cycle as operational unit of neocortical neuronogenesis." Neuroscientist. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Caviness, VS,Jr., Takahashi, T and Nowakowski, RS: "The G1 restriction point as critical regulator of neocortical neuronogenesis" J Neurochem Res. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Caviness, VS.Jr.and Takahashi, T: "La cytogenese neocorticale : le gradient proliferatif de I'epithelium pseudostratifie ventriculaire (PVE) et histogenese du cortex cerebral" Percentiel. 3. 5-10 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takahashi, T,Nowakowski, RS and Caviness, VS,Jr.: "Mathematics of neocortical neuronogenesis." Developmental Neuroscience. 19. 17-22 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miyama S,Takahashi T,Nowakowski RS,Caviness, VS,Jr.: "A gradient in the duration of the G1 phase in the murine neocortical proliferative epithelium." Cerebral Cortex. 17. 678-689 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Caviness, VS,Jr., Takahashi T,Miyama, S,Nowakowski, RS and Delalle, I: "Regulation of normal proliferation in the developing cerebrum : potential actions of trophic factors." Exp Neurol. 137. 357-366 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takahashi, T,Miyama, S and Caviness, VS,Jr.: Regulated parameters of cell proliferation in neocortical histogenesis.In, Neural Development.Eds : K.Uyemura, K.Kawamura and T.Yazaki, Springer-Verlag, (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takahashi, T and Caviness, VS Jr.: Neurogenesis and the earliest events of neocortical histogenesis.In : New developments in child neurology, Ed : Velickovic M,Monduzzi Editore International Proceedings Division, (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Caviness, VS,Jr., Takahashi, T and Nowakowski, RS: Cell Proliferation in cortical development.In : Normal and ebnormal development of the cortex.Eds.Galaburda AM and Christen Y.Springer-Verlag, (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takahashi.Y,Bhide,PG,Goto,T,Miyama.S and Caviness,VS: "Proliferative behavior of the murine cerebral wall in tissue culture:cell cycle kinetics and checkpoints." Experimental Neurology. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takahashi.T,Nowakowski,RS and Caviness,VS: "Cell cycle as operational unit of neocortical neuronogenesis." Neuroscientist. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Caviness,VS,Jr.,Takahashi.T and Nowakowski.RS: "The G1 restriction point as critical regulator of neocortical neuronogenesis." J Neurochem Res. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Caviness,VS.Jr.and Takahashi.T,: "La cytogenese neocorticale:le gradient proliferatif de I'epithelium pseudostratifie ventriculaire(PVE)et histogenese du cortex cerebral." Percentiel. vol3. 5-10 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Caviness,VS,Jr.,Takahashi,T and Nowakowski,RS: "Cell Proliferation in cortical development.In:Normal and abnormal development of the cortex." Galaburda AM and Christen Y.Springer-Verlag, 199 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takahashi.T,Miyama,S and Caviness,VS,Jr.,: "Regulated parameters of cell proliferation in neocortical histogenesis.In:Neural Development" Eds:K Uyemura,K.Kawamura and T.Yazaki Springer-Verlag, 544 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] TAKAHASHI T.: "Mathematics of neocortical neuronogenesis" Experimental Neuroscience. 19. 17-22 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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