|Budget Amount *help
¥2,700,000 (Direct Cost : ¥2,700,000)
Fiscal Year 1999 : ¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 1998 : ¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 1997 : ¥1,300,000 (Direct Cost : ¥1,300,000)
Our subjects were patients who had been diagnosed with acute mayeloid leukemia (AML), non-Hodgkin's lymphoma (NHL), Hodgkin's disease (HD) and myelodysplastic syndromes (MDS). Peripheral blood (PB) was obtained from the patients at diagnosis, except for the AML patients, who were in continued complete remission. Dendritic cells (DC) were purified from PB by Ficoll density centrifugation, T rosetting, dish adherence and metrizamide density centrifugation. DC function was assessed by their capacity to stimulate allogeneic T cells. Expression of functionally important molecules on CD83-positive DC, I.e., adhesion molecules (CD11a, CD54, CD58), co-stimulatory molecules (CD80, CD86, CD40) and HLA (DR, DP, DQ), was analyzed by two-color flow cytometry. The function of DC from NHL and HD patients varied among patients, but was reduced in most patients when compared with the DC function of normal volunteers (NV). Expression of functionally important molecules on DC did not differ significantly between NV and patients with NHL or HD. DC function of AML patients was at least equal to that of NV. The DC function of most MDS patients was similar to that of NV. Some DC were found to be derived from an abnormal clone in MDS patients when analyzed by fluorescence in situ hybridization.
患者によってばらつきは大きいものの、DC機能に低下を示す例が多かった。一方、DCのadhesion molecules(CD11a, CD54, CD58), costimulatory molecules (CD80, CD86, CD40), HLA(DR, DP,DQ)発現は、正常者と同等であった。
一部の患者でDC機能の低下が見られたが、活性の保たれているものが多かった。Adhesion molecules, costimulatory molecules, HLA発現に大きな異常はなかった。FISH解析によって、DCの一部は腫瘍クローン由来と判定された。