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A study on the effect of static stretch on the expression of NOS in glomerular endothelial cells

Research Project

Project/Area Number 09671180
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionJikei University School of Medicine

Principal Investigator

KAWAMURA Tetsuya  Jikei University School of Medicine Department of Medicine II,Lecturer, 内科学講座・第2, 講師 (20161367)

Co-Investigator(Kenkyū-buntansha) MIYAZAKI Yoichi  Jikei University School of Medicine Department of Medicine II,Instructor, 内科学講座・第2, 助手 (60266690)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsglomerular endothelial cell / static stretch / mecharical strain / nitric oxide / endothelial NOS / vascular endothelial cell / cyclic GMP / brady kinin / MCP-1 / 糸球体硬化 / マクロファージ / メサンギウム細胞 / 細胞外基質 / ICAM-1 / M-CSF
Research Abstract

In the present study, we examined the effect of static stretch to glomerular endothelial cells (GEC) and vascular endothelial cells (VEC) on the expression of nitric oxide synthase (NOS) and the production of nitric oxide (NO) by these cells upon the stimulation by bradykinin.
Northern blot analysis and RT-PCR revealed that mRNA levels for endothelial NOS (eNOS) were not significantly different between the cells with and without exposure to 30-40 % static stretch for up to 48hr. In contrast, it was clearly demonstrated by Western blot analysis that the expression of eNOS protein was down-regulated in VEC exposed to static stretch for 24 hours in a stretch-degree dependent manner. Moreover, 10^<-6> M bradykinin markedly increased cGMP contents in untreated VEC, whereas this effect was completely abolished when VEC were exposed to 30% stretch for 24 hours prior to the stimulation by bradykinin. These results indicate that the expression of eNOS protein may be down-regulated in VEC when they are exposed to a high degree of mechanical stretch, leading to a compromised production of NO in response to eNOS agonists. We are in the process of examining the effect of static stretch to GEC on the production of NO upon the stimulation by bradykinin, in order to elucidate a role of a high degree of mechanical stretch to GEC in the regulation of glomerular microcirculation.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 川村哲也: "薬剤の作用機序からみた蛋白尿 -アンジオテンシン変換酵素阻害薬-" 腎と透析. 1. 71-76 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 川村哲也: "メサンギウム細胞の特殊性" 血管と内皮. 7. 57-62 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 川村哲也: "IgA腎症におけるACE阻害薬の使い方" Medical Practice. 14. 1323-1326 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 川村哲也: "ACE阻害薬とAngiotensinII受容体拮抗薬の尿蛋白減少作用" 臨床成人病. 28. 95-99 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 川村哲也: "私の第一選択薬 -IgA腎症" 治療. 80(増刊号). 488-489 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 川村哲也: "分子腎臓病学実験ノート ; 細胞培養法 -上皮・内皮細胞-" 文光堂, 50-57 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 川村哲也: "Annal Review腎臓1997 ; ACE阻害薬の長期投与効果" 中外医学社, 236-242 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Utsunomiya Y,Imasawa T,Abe A,Hirano K,Kawamura T,Nagasawa R,Mitarai T,Maruyama N,Sakai O: "Bacterial superantigen enhances cytokine production by T-helper lymphocyte subset-2 cells and modifies glomerular lesions in experimental Immunoglobulin A nephropathy." Clin Exp Nephrol. 1. 83-91 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshida H,Kawamura T,Ichikawa I,Sakai O: "Polymorphism of renin-angiotensin system genes in progressive IgA nephropathy." Nephrology. 3(suppl). S719-S723 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sakai T,Kawamura T,Shirasawa T: "Mizoribine improves renal tubulointerstitial fibrosis in unilateral ureteral obstruction." J Urol. 158. 2316-2322 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yokoo T,Utsunomiya Y,Ohashi T,Imasawa T,Kogure T,Futagawa Y,Kawamura T,Eto Y,Hosoya T.: "Inflamed site-specific gene delivery using bone marrow-derived CD11b^+CD18^+vehicle cells in mice." Hum Gene Ther. 9. 1731-1738 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Imasawa T,Utsunomiya Y,Kawamura T,Nagasawa R,Maruyama N,Sakai O: "Evidence suggesting the involvement of hematopoietic stem cells in the pathogenesis of IgA nephropathy." Biochem biophys Res Commun. 249. 605-611 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miyazaki Y,Tsuchida S,Nishimura H,Pope J C,Harris R C,McKanna J M,Inagami t, Hogan B L M,Fogo A,Ichikawa I.: "Angiotensin induces the urinary peristaltic machinery during the perinatal period." J Clin Invest. 102. 1489-1497 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Imasawa Y,Utsunomiya Y,Kawamura T,et al.: "Evideuce suggesting the involvement of hematopoietic stem cells in the pathogenesis of Ig A nephropathy" Biochem.Biophys.Res.Commun.249. 605-611 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 川村哲也,宇都宮保典,他: "IgA腎に対するステロイド治療の効果について-経時的生検例の検討から-" 平成9年度厚生省特定疾患進行性腎障害調査研究班研究業績. 17-20 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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