|Budget Amount *help
¥2,900,000 (Direct Cost : ¥2,900,000)
Fiscal Year 1998 : ¥900,000 (Direct Cost : ¥900,000)
Fiscal Year 1997 : ¥2,000,000 (Direct Cost : ¥2,000,000)
In organ transplantation, graft modification said to be reduce the rejection. Therefore. we clarify whether graft modification such as donor total body irradiation (TBI) or recipient bone marrow transplantation (to the donor) (BM) improve the graft survival in small bowel transplantation.
Inbred rats : BN and LEW were used for a donor or a recipient. before orthotopic total small bowel transplantation, donor TB! or BM was performed. We compared the graft survival and onset of graft-versus-host disease (GVHD).
(1) BN*LEW : In control groups, median survival day (MSD) was 20 days, and 2 of 7 rats showed temporally GVHD, which had long term survival. All rats in control group died of rejection. The graft survival in TBI group and BM group decreased to 12 days, 15.5 days (MSD), respectively. None of rats iii both groups showed GVHD.Histologically the findings of the rejection were more slight iii non-GVHD rats than GVHD rats.
(2)LEW-BN : In control groups, median survival day (MSD) was 29 days, and all rats in control group died of GVHD.The graft survival in TBIgroup improved to 69 days (MSD), and all rats in TB! group died of rejection. The graft survival in BM group was 12 days(MSD). All of rats in BM group showed and died of severe OVID.
Survival of the rats without severe GVHD decreased, because that donor TB! only reduce the OVH - reaction, but not reduce the rejection. Recipients bone marrow transplantation increase the antigenicity of the graft, therefore graft survival was worsened and increased the risk of GVHD.In summary, graft modification such as donor TBI or BM can not reduce the rejection.