| Project/Area Number |
09671342
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
HARADA Nobuhiko Tokyo Women's Medical University, School of Medicine Assistant Professor, 医学部, 助手 (10198921)
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| Co-Investigator(Kenkyū-buntansha) |
IMAIZUMI Toshihide Tokyo Women's Medical University, School of Medicine Assosiate Professor, 医学部, 助教授 (10075377)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | pancreatic cancer / p53 / p21 / WAF1 / prognosis / p21WAF1 / アポトーシス / 膵癌細胞株 |
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| Research Abstract |
Sixty-two resected specimens of human ductal adenocarcinoma of the pancreas were investigated on p53 and p21WAF1 immunohistochemically and genetically by RT-PCR.Also correlation between the expression of p53 or p21WAF1 and the prognosis of the patients were studied. Twenty frozen specimens of pancreatic cancer tissue and 6 pancreatic cancer cell lines were checked up by Westernblot and RT-PCR.In immunohistochemistry, 37% of the patients showed p53 positive and 48% showed p21WAF1 positive. Wild-type p53 significantly correlated not only with p21WAF1 protein expression (p <0.03) but also with WAF1 mRNA expression (p <0.005). The mean survival time was 16 months in the patients with p53(-), 10.5 months in p53(+), 12 months in p2l(-) and 15 months in p2l(+). Both WAF1 protein and mRNA were detected only in the pancreatic cancer cell lines with wild-type p53 but not in those with mutant-p53. In pancreatic cancer, wild-type p53 corresponds significantly to the WAF1 expression and this correlations more closely on the mRNA level than on the protein level.Our data suggest a tendency for the tumors expressing mutant-p53 and not expressing p21WAF1 to grow faster.
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