| Project/Area Number |
09671592
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | KAWASAKI MEDICAL SCHOOL |
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Principal Investigator |
FUJITA Yoshihisa Kawasaki Medical School, Medical Faculty, Associate Professor, 医学部, 助教授 (10144263)
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| Co-Investigator(Kenkyū-buntansha) |
YASUKAWA Takeshi Kawasaki Medical School, Medical Faculty, Faculty Assistant, 医学部, 助手 (10289168)
KIMURA Kenichi Kawasaki Medical School, Medical Faculty, Assistant Professor, 医学部, 講師 (90214874)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | microcirculation / lidocaine / leukocytes / endothelium / adhesion molecules / microvessels / vital microscopy / fluorescence |
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| Research Abstract |
The purpose of the research project was to examine the effects of lidocaine on leukocyte-endothelium interaction in retinal microcirculation. Because visualization of the retinal microvessels and blood cells flowing in these microvessels was not possible by our system consisting of a fluorescence vital microscope and an image analyzer (Argus 20, Hamamatsu Photonics), we performed the studies in hamsters where the microscopic observation chamber was implanted on the dorsal skin fold and in Sprague-Dawley rats using the scrotal striated muscle. Animals were divided into the lidocaine group (n=7) receiving lidocaine infusion 0.3mg/kg/hr and the control group (n=7) receiving the ame volume of saline. Leukocyte adhesion to the post-venular endothelium was attenuated in the animals of the lidocaine group (plasma lidocaine concentration : 6.1 *3.3 mu g/mL) as compared with those of the control group. The number of rolling leukocytes on the post-capillary venules did not differ among the contr
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ol and lidocaine groups. We concluded that attenuation of the leukocyte adhesion to the post-capillary venule may be responsible for the anti-inflammatory property of the lidocaine.
In another study, we tried to visualize the retinal microcirculation in rabbits by using Scanning Laser Ophthalmoscopy (SLO, Rodenstock, Germany). Rabbits with ligation of bilateral carotid artery were anesthetized either with propofol or halothane. The effects of induced hypotension (mean arterial pressure=ca. 50 mmHg) by these anesthetics on retinal microcirculation were compared. Capillary leakage or blockage was observed in neither group, suggesting the safety of propofol anesthesia in ophthalmologic surgery.
Although the system permits visualization and quantitative analysis of retinal inicrovessels and their plasma flow by using fluorescein sodium, it could not visualize leukocytes in the retinal microcirculation with rhodamine 6G, a fluorescent dye, which is used for staining leukocytes in microcirculation research. Advances in Laser technology or staining techniques are necessary for investigation of leukocyte hemodynamics in the retinal microcirculation. Less
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