|Budget Amount *help
¥3,200,000 (Direct Cost : ¥3,200,000)
Fiscal Year 1998 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 1997 : ¥2,700,000 (Direct Cost : ¥2,700,000)
INTRODUCTION AND OBJECTIVES : In normal renal tubules, E-or N-cadherin (CD) is considered to function as an intercellular adhesion complex, adherens junction (AJ). Recently, cDNA sequence of K-CD, which exists in rat kidney, was proved to be identical to that of human CD-6. In this study, we investigated the expression profile of the AJ components, ODs (E, N, P-CD, CD-6) and catenins (alpha, beta, gamma), in established renal cancer (RCC) cell lines.
METHODS : Seven RCC cell lines were investigated with reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting (WB) and immunofluorescence staining (IF). Single strand conformational polymorphism (PCR-SSCP) was examined for beta-catenin.
RESULTS : Normal mRNA of each CD and catenin except P-CD was detected in all cell lines. One of two cell lines with islet-forming growth pattern, KRC/Y, had normal expression of E-CD protein, whereas another cell line, A704, showed weak expression of E-and N-CD.Most cell lines with loose cell compaction showed weak to no expression of both CDs. mRNA of CD-6 was detected in 6 of 7 cell lines except ACTH, a cell line with loose cell compaction. Protein expression of each catenin was conserved in most cell lines with some variations of amount. PCR-SSCP revealed no mutation of beta-catenin.
CONCLUSIONS : These results suggest that the CD function in RCC may be controlled by expression level of E- or N-CD protein, rather than catenins. Beside E-CD, more than one member of CD family including CD-6 may contribute to the compact growing pattern.