Functional analysis of protein phosphatase in vestibulo-cereberum induced by unitateral labyrinthectomy in rats

• Project/Area Number: 09671743
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Osaka University
• Principal Investigator: TAKEDA Noriaki Osaka University Medical School, Associate Professor, 医学部, 助教授 (30206982)
• Co-Investigator(Kenkyū-buntansha): KIYAMA Hiroshi Asahikawa Medical College, Professor, 教授 (00192021)
• Project Period (FY): 1997 – 1998
• Project Status: Completed (Fiscal Year 1998)
• Budget Amount: ¥3,600,000 (Direct Cost: ¥3,600,000); Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 1997: ¥2,900,000 (Direct Cost: ¥2,900,000)
• Keywords: differential display / labyrinthectomy / vestibular compensation / protein phosphatase / glutamate receptor / グルタミン酸レセプター

Research Abstract

The differential desplay method was used to identify the gene expression which was altered in the cerebellar flocculus after unilateral labyrintyectomy (UL).Total RNA from flocculi of sham-operated and labyrinthectomized rats was isolated, amplified by PCR using arbitrary primer sets and separated by electrophoresis on a polyacrylarmide gel.PCR products, of which amounts were significantly different in samples from labyrinthectomized rats and those from controls, were cut out of the gel and sequenced.One of the up-regulated products was the rat protein phosphatase 2A beta catalytic subunit mRNA and one of the down-regulated products was the rat glutamate receptor delta-2 subunit mRNA.Ilistochemical study of in situ hybridization showed that those molecules were localized in the Purkinje cell layer.In labyrinthectomized rats, UL-induced nystagmus gradually disappeared within 3days after UL.These findings suggested that changes in expression of those molecules in the floccular Purkinje cells after UL is involved in vestibular compensation.

Research Products

• [Publications] Kitahara T,Takeda N,et al.: "Unilateral labyrinthectomy downregulates glutamate receptor δ-2 expression in the rat vestibulocerebellum." Mol.Brain Res.61. 170-179 (1998)
• [Publications] Kitahara T,Takeda N,et al.: "An implication of protein phosphatase 2A-β in rat flocculus for lesion-induced vestibular plasticity." Acta Otolaryngol.(Stockh.). 118. 685-691 (1998)
• [Publications] 北原 糺、武田憲昭、他: "分子生物学的手法による前庭代償分子機構の解明:Differential Display 法を用いて" 日耳鼻. 101. 37-43 (1998)
• [Publications] 北原 糺、武田憲昭、他: "前庭小脳グルタミン酸受容体デルタ2の前庭代償への関与" 頭頚部自律神経. 12. 33-35 (1998)
• [Publications] 北原糺、武田憲昭、他: "前庭代償における小脳プロテイン・フォスファターゼ2Aの関与・Differential Displayを用いた前庭代償関連因子の検索" 頭頚部自律神経. 11. 8-10 (1997)
• [Publications] Kitahara T, Takeda N, et al.: "Role of the flocculus for the development of vestibular compensation" Neuroscience. 76. 571-580 (1997)
• [Publications] 武田憲昭、北原糺: "前庭代償" Equilibrium Res.56. 306-307 (1997)
• [Publications] Nishiike S, Takeda N, et al.: "Neurons in rostral ventrolateral medulla mediate vestibular inhibition of locus coeruleus in rats." Neuroscience. 77. 219-232 (1997)
• [Publications] Uno A, Takeda N, et al.: "Histamine release from the hypothalamus induced by changes in gravity in rats and its relation to space adaptation syndrome." Physiol.Behav.61. 883-887 (1997)
• [Publications] Kitahara T, Takeda N, et al.: "Changes in nitric oxide synthetase-like immunoreactivities in unipolar cells in the rat cerebellar flocculus after unilateral labyrinthectomy." Brain Res.765. 1-6 (1997)