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Control of fibroblast growth after glaucoma filtration surgery by neutralizing monoclonal antibody against growth factors.

Research Project

Project/Area Number 09671822
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionKanazawa Medical University

Principal Investigator

NISHIKAWA Katsuzo  Kanazawa Medical University, Medical School, Professor, 医学部, 教授 (10029960)

Co-Investigator(Kenkyū-buntansha) FUKUDA Masamichi  Kanazawa Medical University, Medical School, Lecturer, 医学部, 助手 (70208966)
TAKAHASHI Nobuo  Kanazawa Medical University, Medical Research Institute, Professor, 総合医学研究所, 教授 (20006787)
TAKEUCHI Fumito  Kanazawa Medical University, Medical School, Assistant Professor, 医学部, 講師 (70262623)
YOSHITAKE Yoshino  Kanazawa Medical University, Medical School, Assistant Professor, 医学部, 講師 (00150764)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsglaucoma / growth factor / fibroblasts / monoclonal antibody / growth inhibition / fibroblast growth factor
Research Abstract

The formation of fibrocellular scar decides failure of glaucoma filtration surgery. Tenon's capsule fibroblasts are the main components of the scar. The experiments were made to obtain greater understanding the pathophysilogy of the scar formation by studying the roles of growth factors such as basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) in the growth of human Tenon's fibroblasts.
Growth of fibroblast cell line established from primary culture of human Tenon's capsule was stimulated by bFGF or EGF in the presence of various concentrations of fetal bovine serum (FBS) in dose-dependent fashion. Strong immuno-fluorescence staining of bFGF was observed in nuclei of the cells, but EGF was not detected. bFGF content in extract of the cells was determined to be 270 ng/lO7cells by sandwich radioimmunoassay with 125I-labeled monoclonal antibody against bFGF and heparin-Sepharose. The expression of bFGF mRNA was also observed in the cultured fibroblasts by RT-PCR method using the specific primers for bFGF.The expression was down-regulated by the addition of bFGF in the medium.
When the fibroblasts were incubated for few days in culture medium supplemented with neutralizing monoclonal antibody against bFGF but without bFGF, viability as measured by the number of colonies formed in the medium without the antibody 1 week later was decreased, indicating that secreted endogenous bFGF acts as a survival factor for these cells. The functional roles of bFGF localized in nuclei of the cultured Tenon's capsule fibroblasts are still unclear.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Y.Otsuka: "Requirement of fibroblast growth factor signaling for regeneration of epi-physeal morphology in rabbit full-thikness defects of articular cartilage." Develop.Growth Differ.39. 143-156 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] N.Takahashi: "Expression and function of growth factors in cultured fibroblasts from human Tenon's capsule." Ophthalmic Res.in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nobuo Takahashi, Masamichi Fukuda, Madoka Oda, Takako Nakamura, Yoshino Yoshitake, and Katsuzo Nishikawa: "Expression and function of growth factors in cultured fibroblasts from human Tenon's capsule." Ophthalmic Res.(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Otsuka: "Requirement of fibroblast growth factor signaling for regeneration of epiphyseal morphology in rabbit fullthikness defects of articular cartilage" Develop.Growth Differ.39. 143-156 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] P.J.Dempsey: "Apical enrichment of human EGF precursor in MDCK cells involves preferential basolateral ectodomain cleavage sensitive to a metalloprotease inhibitor" J.Cell Biol.138. 747-758 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] A.Yamakawa: "cDNA cloning and chromosomal mapping of genes encoding novel protein kinases termed PKU-a and PKU-b,which have nuclear localization signal" Gene. 202. 193-201 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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